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Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock

Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafis...

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Autores principales: Lleras Forero, Laura, Narayanan, Rachna, Huitema, Leonie FA, VanBergen, Maaike, Apschner, Alexander, Peterson-Maduro, Josi, Logister, Ive, Valentin, Guillaume, Morelli, Luis G, Oates, Andrew C, Schulte-Merker, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962341/
https://www.ncbi.nlm.nih.gov/pubmed/29624170
http://dx.doi.org/10.7554/eLife.33843
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author Lleras Forero, Laura
Narayanan, Rachna
Huitema, Leonie FA
VanBergen, Maaike
Apschner, Alexander
Peterson-Maduro, Josi
Logister, Ive
Valentin, Guillaume
Morelli, Luis G
Oates, Andrew C
Schulte-Merker, Stefan
author_facet Lleras Forero, Laura
Narayanan, Rachna
Huitema, Leonie FA
VanBergen, Maaike
Apschner, Alexander
Peterson-Maduro, Josi
Logister, Ive
Valentin, Guillaume
Morelli, Luis G
Oates, Andrew C
Schulte-Merker, Stefan
author_sort Lleras Forero, Laura
collection PubMed
description Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord.
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spelling pubmed-59623412018-05-24 Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock Lleras Forero, Laura Narayanan, Rachna Huitema, Leonie FA VanBergen, Maaike Apschner, Alexander Peterson-Maduro, Josi Logister, Ive Valentin, Guillaume Morelli, Luis G Oates, Andrew C Schulte-Merker, Stefan eLife Developmental Biology Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord. eLife Sciences Publications, Ltd 2018-04-06 /pmc/articles/PMC5962341/ /pubmed/29624170 http://dx.doi.org/10.7554/eLife.33843 Text en © 2018, Lleras Forero et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology
Lleras Forero, Laura
Narayanan, Rachna
Huitema, Leonie FA
VanBergen, Maaike
Apschner, Alexander
Peterson-Maduro, Josi
Logister, Ive
Valentin, Guillaume
Morelli, Luis G
Oates, Andrew C
Schulte-Merker, Stefan
Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
title Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
title_full Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
title_fullStr Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
title_full_unstemmed Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
title_short Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
title_sort segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962341/
https://www.ncbi.nlm.nih.gov/pubmed/29624170
http://dx.doi.org/10.7554/eLife.33843
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