Cargando…
Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafis...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962341/ https://www.ncbi.nlm.nih.gov/pubmed/29624170 http://dx.doi.org/10.7554/eLife.33843 |
_version_ | 1783324877212614656 |
---|---|
author | Lleras Forero, Laura Narayanan, Rachna Huitema, Leonie FA VanBergen, Maaike Apschner, Alexander Peterson-Maduro, Josi Logister, Ive Valentin, Guillaume Morelli, Luis G Oates, Andrew C Schulte-Merker, Stefan |
author_facet | Lleras Forero, Laura Narayanan, Rachna Huitema, Leonie FA VanBergen, Maaike Apschner, Alexander Peterson-Maduro, Josi Logister, Ive Valentin, Guillaume Morelli, Luis G Oates, Andrew C Schulte-Merker, Stefan |
author_sort | Lleras Forero, Laura |
collection | PubMed |
description | Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord. |
format | Online Article Text |
id | pubmed-5962341 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59623412018-05-24 Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock Lleras Forero, Laura Narayanan, Rachna Huitema, Leonie FA VanBergen, Maaike Apschner, Alexander Peterson-Maduro, Josi Logister, Ive Valentin, Guillaume Morelli, Luis G Oates, Andrew C Schulte-Merker, Stefan eLife Developmental Biology Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord. eLife Sciences Publications, Ltd 2018-04-06 /pmc/articles/PMC5962341/ /pubmed/29624170 http://dx.doi.org/10.7554/eLife.33843 Text en © 2018, Lleras Forero et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology Lleras Forero, Laura Narayanan, Rachna Huitema, Leonie FA VanBergen, Maaike Apschner, Alexander Peterson-Maduro, Josi Logister, Ive Valentin, Guillaume Morelli, Luis G Oates, Andrew C Schulte-Merker, Stefan Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
title | Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
title_full | Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
title_fullStr | Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
title_full_unstemmed | Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
title_short | Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
title_sort | segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
topic | Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962341/ https://www.ncbi.nlm.nih.gov/pubmed/29624170 http://dx.doi.org/10.7554/eLife.33843 |
work_keys_str_mv | AT llerasforerolaura segmentationofthezebrafishaxialskeletonreliesonnotochordsheathcellsandnotonthesegmentationclock AT narayananrachna segmentationofthezebrafishaxialskeletonreliesonnotochordsheathcellsandnotonthesegmentationclock AT huitemaleoniefa segmentationofthezebrafishaxialskeletonreliesonnotochordsheathcellsandnotonthesegmentationclock AT vanbergenmaaike segmentationofthezebrafishaxialskeletonreliesonnotochordsheathcellsandnotonthesegmentationclock AT apschneralexander segmentationofthezebrafishaxialskeletonreliesonnotochordsheathcellsandnotonthesegmentationclock AT petersonmadurojosi segmentationofthezebrafishaxialskeletonreliesonnotochordsheathcellsandnotonthesegmentationclock AT logisterive segmentationofthezebrafishaxialskeletonreliesonnotochordsheathcellsandnotonthesegmentationclock AT valentinguillaume segmentationofthezebrafishaxialskeletonreliesonnotochordsheathcellsandnotonthesegmentationclock AT morelliluisg segmentationofthezebrafishaxialskeletonreliesonnotochordsheathcellsandnotonthesegmentationclock AT oatesandrewc segmentationofthezebrafishaxialskeletonreliesonnotochordsheathcellsandnotonthesegmentationclock AT schultemerkerstefan segmentationofthezebrafishaxialskeletonreliesonnotochordsheathcellsandnotonthesegmentationclock |