Using Single Molecule mRNA Fluorescent in Situ Hybridization (RNA-FISH) to Quantify mRNAs in Individual Murine Oocytes and Embryos

Changes in abundance of mRNAs during oocyte growth and maturation and during pre-implantation embryo development have been documented using quantitative real-time RT-PCR (qPCR), microarray analyses, and whole genome sequencing. However, these techniques require amplification of mRNAs, normalization...

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Detalles Bibliográficos
Autores principales: Xie, Fang, Timme, Kelsey A., Wood, Jennifer R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962540/
https://www.ncbi.nlm.nih.gov/pubmed/29785002
http://dx.doi.org/10.1038/s41598-018-26345-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962540/
https://www.ncbi.nlm.nih.gov/pubmed/29785002
http://dx.doi.org/10.1038/s41598-018-26345-0

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