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Using Single Molecule mRNA Fluorescent in Situ Hybridization (RNA-FISH) to Quantify mRNAs in Individual Murine Oocytes and Embryos

Changes in abundance of mRNAs during oocyte growth and maturation and during pre-implantation embryo development have been documented using quantitative real-time RT-PCR (qPCR), microarray analyses, and whole genome sequencing. However, these techniques require amplification of mRNAs, normalization...

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Detalles Bibliográficos
Autores principales:	Xie, Fang, Timme, Kelsey A., Wood, Jennifer R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962540/ https://www.ncbi.nlm.nih.gov/pubmed/29785002 http://dx.doi.org/10.1038/s41598-018-26345-0

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