Simultaneous cell disruption and semi-quantitative activity assays for high-throughput screening of thermostable L-asparaginases

L-asparaginase, which catalyses the hydrolysis of L-asparagine to L-aspartate, has attracted the attention of researchers due to its expanded applications in medicine and the food industry. In this study, a novel thermostable L-asparaginase from Pyrococcus yayanosii CH1 was cloned and over-expressed...

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Detalles Bibliográficos
Autores principales: Li, Xu, Zhang, Xian, Xu, Shuqin, Zhang, Hengwei, Xu, Meijuan, Yang, Taowei, Wang, Li, Qian, Haifeng, Zhang, Huiling, Fang, Haitian, Osire, Tolbert, Rao, Zhiming, Yang, Shangtian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962637/
https://www.ncbi.nlm.nih.gov/pubmed/29784948
http://dx.doi.org/10.1038/s41598-018-26241-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962637/
https://www.ncbi.nlm.nih.gov/pubmed/29784948
http://dx.doi.org/10.1038/s41598-018-26241-7

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