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Simultaneous cell disruption and semi-quantitative activity assays for high-throughput screening of thermostable L-asparaginases

L-asparaginase, which catalyses the hydrolysis of L-asparagine to L-aspartate, has attracted the attention of researchers due to its expanded applications in medicine and the food industry. In this study, a novel thermostable L-asparaginase from Pyrococcus yayanosii CH1 was cloned and over-expressed...

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Detalles Bibliográficos
Autores principales:	Li, Xu, Zhang, Xian, Xu, Shuqin, Zhang, Hengwei, Xu, Meijuan, Yang, Taowei, Wang, Li, Qian, Haifeng, Zhang, Huiling, Fang, Haitian, Osire, Tolbert, Rao, Zhiming, Yang, Shangtian
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962637/ https://www.ncbi.nlm.nih.gov/pubmed/29784948 http://dx.doi.org/10.1038/s41598-018-26241-7

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