A Modified Monomeric Red Fluorescent Protein Reporter for Assessing CRISPR Activity

Gene editing in human embryonic stem cells (hESCs) has been significantly enhanced by the discovery and development of CRISPR Cas9, a programmable nuclease system that can introduce targeted double-stranded breaks. The system relies on the optimal selection of a sgRNA sequence with low off-targets a...

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Detalles Bibliográficos
Autores principales: Højland Knudsen, Camilla, Ásgrímsdóttir, Emilía S., Rahimi, Karim, Gill, Katherine P., Frandsen, Søs, Hvolbøl Buchholdt, Susanne, Chen, Muwan, Kjems, Jørgen, Febbraro, Fabia, Denham, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Cell and Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962823/
https://www.ncbi.nlm.nih.gov/pubmed/29868584
http://dx.doi.org/10.3389/fcell.2018.00054

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962823/
https://www.ncbi.nlm.nih.gov/pubmed/29868584
http://dx.doi.org/10.3389/fcell.2018.00054

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