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Direct multiplex imaging and optogenetics of RhoGTPases enabled by near-infrared FRET

Direct visualization and light control of several cellular processes is a challenge due to spectral overlap of available genetically-encoded probes. Here we report the most red-shifted monomeric near-infrared (NIR) fluorescent protein miRFP720 and the fully NIR Förster Resonance Energy Transfer (FRE...

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Detalles Bibliográficos
Autores principales: Shcherbakova, Daria M., Cammer, Natasha Cox, Huisman, Tsipora M., Verkhusha, Vladislav V., Hodgson, Louis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5964015/
https://www.ncbi.nlm.nih.gov/pubmed/29686359
http://dx.doi.org/10.1038/s41589-018-0044-1