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Functional analysis of eliciting plant response protein Epl1-Tas from Trichoderma asperellum ACCC30536

Eliciting plant response protein (Epl) is a small Trichoderma secreted protein that acts as an elicitor to induce plant defense responses against pathogens. In the present study, the differential expression, promoter analysis, and phylogenetic tree analysis of Epl1-Tas (GenBank JN966996) from T. asp...

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Autores principales: Yu, Wenjing, Mijiti, Gulijimila, Huang, Ying, Fan, Haijuan, Wang, Yucheng, Liu, Zhihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5964103/
https://www.ncbi.nlm.nih.gov/pubmed/29789617
http://dx.doi.org/10.1038/s41598-018-26328-1
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author Yu, Wenjing
Mijiti, Gulijimila
Huang, Ying
Fan, Haijuan
Wang, Yucheng
Liu, Zhihua
author_facet Yu, Wenjing
Mijiti, Gulijimila
Huang, Ying
Fan, Haijuan
Wang, Yucheng
Liu, Zhihua
author_sort Yu, Wenjing
collection PubMed
description Eliciting plant response protein (Epl) is a small Trichoderma secreted protein that acts as an elicitor to induce plant defense responses against pathogens. In the present study, the differential expression, promoter analysis, and phylogenetic tree analysis of Epl1-Tas (GenBank JN966996) from T. asperellum ACCC30536 were performed. The results showed Epl1-Tas could play an important role in the interaction between T. asperellum ACCC30536 and woody plant or woody plant pathogen. Furthermore, the effect of the Escherichia coli recombinant protein rEpl1-e and the Pichia pastoris recombinant protein rEpl1-p on Populus davidiana × P. alba var. pyramidalis (PdPap) was studied. In PdPap seedlings, rEpl1-e or rEpl1-p induction altered the expression levels of 11 genes in the salicylic acid (SA, three genes), jasmonic acid (JA, four genes) and auxin (four genes) signal transduction pathways, and five kinds of enzymes activities The induction level of rEpl1-p was significantly higher than that of rEpl1-e, indicating that rEpl1-p could be used for further induction experiment. Under 3 mg/mL rEpl1-p induction, the mean height of the PdPap seedlings increased by 57.65% and the mean lesion area on the PdPap seedlings leaves challenged with Alternaria alternata decreased by 91.22% compared with those of the control. Thus, elicitor Epl1-Tas could induce the woody plant resistance to pathogen.
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spelling pubmed-59641032018-05-24 Functional analysis of eliciting plant response protein Epl1-Tas from Trichoderma asperellum ACCC30536 Yu, Wenjing Mijiti, Gulijimila Huang, Ying Fan, Haijuan Wang, Yucheng Liu, Zhihua Sci Rep Article Eliciting plant response protein (Epl) is a small Trichoderma secreted protein that acts as an elicitor to induce plant defense responses against pathogens. In the present study, the differential expression, promoter analysis, and phylogenetic tree analysis of Epl1-Tas (GenBank JN966996) from T. asperellum ACCC30536 were performed. The results showed Epl1-Tas could play an important role in the interaction between T. asperellum ACCC30536 and woody plant or woody plant pathogen. Furthermore, the effect of the Escherichia coli recombinant protein rEpl1-e and the Pichia pastoris recombinant protein rEpl1-p on Populus davidiana × P. alba var. pyramidalis (PdPap) was studied. In PdPap seedlings, rEpl1-e or rEpl1-p induction altered the expression levels of 11 genes in the salicylic acid (SA, three genes), jasmonic acid (JA, four genes) and auxin (four genes) signal transduction pathways, and five kinds of enzymes activities The induction level of rEpl1-p was significantly higher than that of rEpl1-e, indicating that rEpl1-p could be used for further induction experiment. Under 3 mg/mL rEpl1-p induction, the mean height of the PdPap seedlings increased by 57.65% and the mean lesion area on the PdPap seedlings leaves challenged with Alternaria alternata decreased by 91.22% compared with those of the control. Thus, elicitor Epl1-Tas could induce the woody plant resistance to pathogen. Nature Publishing Group UK 2018-05-22 /pmc/articles/PMC5964103/ /pubmed/29789617 http://dx.doi.org/10.1038/s41598-018-26328-1 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yu, Wenjing
Mijiti, Gulijimila
Huang, Ying
Fan, Haijuan
Wang, Yucheng
Liu, Zhihua
Functional analysis of eliciting plant response protein Epl1-Tas from Trichoderma asperellum ACCC30536
title Functional analysis of eliciting plant response protein Epl1-Tas from Trichoderma asperellum ACCC30536
title_full Functional analysis of eliciting plant response protein Epl1-Tas from Trichoderma asperellum ACCC30536
title_fullStr Functional analysis of eliciting plant response protein Epl1-Tas from Trichoderma asperellum ACCC30536
title_full_unstemmed Functional analysis of eliciting plant response protein Epl1-Tas from Trichoderma asperellum ACCC30536
title_short Functional analysis of eliciting plant response protein Epl1-Tas from Trichoderma asperellum ACCC30536
title_sort functional analysis of eliciting plant response protein epl1-tas from trichoderma asperellum accc30536
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5964103/
https://www.ncbi.nlm.nih.gov/pubmed/29789617
http://dx.doi.org/10.1038/s41598-018-26328-1
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