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TGF-β concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration
Controversy still exists regarding the role of the TGF-β in neovascular age-related macular degeneration (nAMD), a major cause of severe visual loss in the elderly in developed countries. Here, we measured the concentrations of active TGF-β1, TGF-β2, and TGF-β3 by ELISA in the aqueous humor of 20 pa...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5966430/ https://www.ncbi.nlm.nih.gov/pubmed/29795291 http://dx.doi.org/10.1038/s41598-018-26442-0 |
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author | Tosi, Gian Marco Neri, Giovanni Caldi, Elena Fusco, Fiorella Bacci, Tommaso Tarantello, Antonio Nuti, Elisabetta Marigliani, Davide Baiocchi, Stefano Traversi, Claudio Barbarino, Marcella Eandi, Chiara M. Parolini, Barbara Mundo, Lucia Santucci, Annalisa Orlandini, Maurizio Galvagni, Federico |
author_facet | Tosi, Gian Marco Neri, Giovanni Caldi, Elena Fusco, Fiorella Bacci, Tommaso Tarantello, Antonio Nuti, Elisabetta Marigliani, Davide Baiocchi, Stefano Traversi, Claudio Barbarino, Marcella Eandi, Chiara M. Parolini, Barbara Mundo, Lucia Santucci, Annalisa Orlandini, Maurizio Galvagni, Federico |
author_sort | Tosi, Gian Marco |
collection | PubMed |
description | Controversy still exists regarding the role of the TGF-β in neovascular age-related macular degeneration (nAMD), a major cause of severe visual loss in the elderly in developed countries. Here, we measured the concentrations of active TGF-β1, TGF-β2, and TGF-β3 by ELISA in the aqueous humor of 20 patients affected by nAMD, who received 3 consecutive monthly intravitreal injections of anti-VEGF-A antibody. Samples were collected at baseline (before the first injection), month 1 (before the second injection), and month 2 (before the third injection). The same samples were used in a luciferase-based reporter assay to test the TGF-β pathway activation. Active TGF-β1 concentrations in the aqueous humor were below the minimum detectable dose. Active TGF-β2 concentrations were significantly lower at baseline and at month 1, compared to controls. No significant differences in active TGF-β3 concentration were found among the sample groups. Moreover, TGF-β pathway activation was significantly lower at baseline compared to controls. Our data corroborate an anti-angiogenic role for TGF-β2 in nAMD. This should be considered from the perspective of a therapy using TGF-β inhibitors. |
format | Online Article Text |
id | pubmed-5966430 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59664302018-05-24 TGF-β concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration Tosi, Gian Marco Neri, Giovanni Caldi, Elena Fusco, Fiorella Bacci, Tommaso Tarantello, Antonio Nuti, Elisabetta Marigliani, Davide Baiocchi, Stefano Traversi, Claudio Barbarino, Marcella Eandi, Chiara M. Parolini, Barbara Mundo, Lucia Santucci, Annalisa Orlandini, Maurizio Galvagni, Federico Sci Rep Article Controversy still exists regarding the role of the TGF-β in neovascular age-related macular degeneration (nAMD), a major cause of severe visual loss in the elderly in developed countries. Here, we measured the concentrations of active TGF-β1, TGF-β2, and TGF-β3 by ELISA in the aqueous humor of 20 patients affected by nAMD, who received 3 consecutive monthly intravitreal injections of anti-VEGF-A antibody. Samples were collected at baseline (before the first injection), month 1 (before the second injection), and month 2 (before the third injection). The same samples were used in a luciferase-based reporter assay to test the TGF-β pathway activation. Active TGF-β1 concentrations in the aqueous humor were below the minimum detectable dose. Active TGF-β2 concentrations were significantly lower at baseline and at month 1, compared to controls. No significant differences in active TGF-β3 concentration were found among the sample groups. Moreover, TGF-β pathway activation was significantly lower at baseline compared to controls. Our data corroborate an anti-angiogenic role for TGF-β2 in nAMD. This should be considered from the perspective of a therapy using TGF-β inhibitors. Nature Publishing Group UK 2018-05-23 /pmc/articles/PMC5966430/ /pubmed/29795291 http://dx.doi.org/10.1038/s41598-018-26442-0 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Tosi, Gian Marco Neri, Giovanni Caldi, Elena Fusco, Fiorella Bacci, Tommaso Tarantello, Antonio Nuti, Elisabetta Marigliani, Davide Baiocchi, Stefano Traversi, Claudio Barbarino, Marcella Eandi, Chiara M. Parolini, Barbara Mundo, Lucia Santucci, Annalisa Orlandini, Maurizio Galvagni, Federico TGF-β concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration |
title | TGF-β concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration |
title_full | TGF-β concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration |
title_fullStr | TGF-β concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration |
title_full_unstemmed | TGF-β concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration |
title_short | TGF-β concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration |
title_sort | tgf-β concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5966430/ https://www.ncbi.nlm.nih.gov/pubmed/29795291 http://dx.doi.org/10.1038/s41598-018-26442-0 |
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