Uncoupling of sgRNAs from their associated barcodes during PCR amplification of combinatorial CRISPR screens

Many implementations of pooled screens in mammalian cells rely on linking an element of interest to a barcode, with the latter subsequently quantitated by next generation sequencing. However, substantial uncoupling between these paired elements during lentiviral production has been reported, especia...

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Detalles Bibliográficos
Autores principales: Hegde, Mudra, Strand, Christine, Hanna, Ruth E., Doench, John G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5969736/
https://www.ncbi.nlm.nih.gov/pubmed/29799876
http://dx.doi.org/10.1371/journal.pone.0197547

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5969736/
https://www.ncbi.nlm.nih.gov/pubmed/29799876
http://dx.doi.org/10.1371/journal.pone.0197547

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