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Uncoupling of sgRNAs from their associated barcodes during PCR amplification of combinatorial CRISPR screens
Many implementations of pooled screens in mammalian cells rely on linking an element of interest to a barcode, with the latter subsequently quantitated by next generation sequencing. However, substantial uncoupling between these paired elements during lentiviral production has been reported, especia...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5969736/ https://www.ncbi.nlm.nih.gov/pubmed/29799876 http://dx.doi.org/10.1371/journal.pone.0197547 |