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An improved primer set and amplification protocol with increased specificity and sensitivity targeting the Symbiodinium ITS2 region

The Internal Transcribed Spacer 2 (ITS2) rRNA gene is a commonly targeted genetic marker to assess diversity of Symbiodinium, a dinoflagellate genus of algal endosymbionts that is pervasively associated with marine invertebrates, and notably reef-building corals. Here we tested three commonly used I...

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Detalles Bibliográficos
Autores principales: Hume, Benjamin C.C., Ziegler, Maren, Poulain, Julie, Pochon, Xavier, Romac, Sarah, Boissin, Emilie, de Vargas, Colomban, Planes, Serge, Wincker, Patrick, Voolstra, Christian R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5970565/
https://www.ncbi.nlm.nih.gov/pubmed/29844969
http://dx.doi.org/10.7717/peerj.4816