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Development of a single-tube one-step RT-LAMP assay to detect the Chikungunya virus genome

BACKGROUND: A single-tube one-step real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of chikungunya virus (CHIKV) targeting the conserved 6K-E1 target region was developed. The assay was validated with sera collected from a CHIKV outbreak in S...

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Detalles Bibliográficos
Autores principales: Lopez-Jimena, Benjamin, Wehner, Stefanie, Harold, Graham, Bakheit, Mohammed, Frischmann, Sieghard, Bekaert, Michaël, Faye, Oumar, Sall, Amadou Alpha, Weidmann, Manfred
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5973553/
https://www.ncbi.nlm.nih.gov/pubmed/29813065
http://dx.doi.org/10.1371/journal.pntd.0006448