Cargando…

Nanopore sequencing of drug-resistance-associated genes in malaria parasites, Plasmodium falciparum

Here, we report the application of a portable sequencer, MinION, for genotyping the malaria parasite Plasmodium falciparum. In the present study, an amplicon mixture of nine representative genes causing resistance to anti-malaria drugs is diagnosed. First, we developed the procedure for four laborat...

Descripción completa

Detalles Bibliográficos
Autores principales:	Runtuwene, Lucky R., Tuda, Josef S. B., Mongan, Arthur E., Makalowski, Wojciech, Frith, Martin C., Imwong, Mallika, Srisutham, Suttipat, Nguyen Thi, Lan Anh, Tuan, Nghia Nguyen, Eshita, Yuki, Maeda, Ryuichiro, Yamagishi, Junya, Suzuki, Yutaka
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5974085/ https://www.ncbi.nlm.nih.gov/pubmed/29844487 http://dx.doi.org/10.1038/s41598-018-26334-3

Ejemplares similares

An innovative diagnostic technology for the codon mutation C580Y in kelch13 of Plasmodium falciparum with MinION nanopore sequencer
por: Imai, Kazuo, et al.
Publicado: (2018)

Interactive transcriptome analysis of malaria patients and infecting Plasmodium falciparum
por: Yamagishi, Junya, et al.
Publicado: (2014)

Genetic polymorphisms in Plasmodium falciparum chloroquine resistance genes, pfcrt and pfmdr1, in North Sulawesi, Indonesia
por: Reteng, Patrick, et al.
Publicado: (2017)

A novel diagnostic method for malaria using loop-mediated isothermal amplification (LAMP) and MinION™ nanopore sequencer
por: Imai, Kazuo, et al.
Publicado: (2017)

Measurement of gene amplifications related to drug resistance in Plasmodium falciparum using droplet digital PCR
por: Srisutham, Suttipat, et al.
Publicado: (2021)

Serotyping dengue virus with isothermal amplification and a portable sequencer
por: Yamagishi, Junya, et al.
Publicado: (2017)

Global research alliance in infectious disease: a collaborative effort to combat infectious diseases through dissemination of portable sequencing
por: Runtuwene, Lucky R., et al.
Publicado: (2022)

Four human Plasmodium species quantification using droplet digital PCR
por: Srisutham, Suttipat, et al.
Publicado: (2017)

Utility of Plasmodium falciparum DNA from rapid diagnostic test kits for molecular analysis and whole genome amplification
por: Srisutham, Suttipat, et al.
Publicado: (2020)

Portable sequencer in the fight against infectious disease
por: Mongan, Arthur Elia, et al.
Publicado: (2019)

Molecular surveillance for operationally relevant genetic polymorphisms in Plasmodium falciparum in Southern Chad, 2016–2017
por: Das, Sukanta, et al.
Publicado: (2022)

A novel sensitive hexaplex high-resolution melt assay for identification of five human Plasmodium species plus internal control
por: Srisutham, Suttipat, et al.
Publicado: (2023)

Genetic population of Plasmodium knowlesi during pre-malaria elimination in Thailand
por: Sugaram, Rungniran, et al.
Publicado: (2021)

DB-AT: a 2015 update to the Full-parasites database brings a multitude of new transcriptomic data for apicomplexan parasites
por: Jąkalski, Marcin, et al.
Publicado: (2014)

Genetic diversity of three surface protein genes in Plasmodium malariae from three Asian countries
por: Srisutham, Suttipat, et al.
Publicado: (2018)

An online mapping database of molecular markers of drug resistance in Plasmodium falciparum: the ACT Partner Drug Molecular Surveyor
por: Otienoburu, Sabina Dahlström, et al.
Publicado: (2019)

Ten-year persistence and evolution of Plasmodium falciparum antifolate and anti-sulfonamide resistance markers pfdhfr and pfdhps in three Asian countries
por: Srisutham, Suttipat, et al.
Publicado: (2022)

The Lethal(2)-Essential-for-Life [L(2)EFL] Gene Family Modulates Dengue Virus Infection in Aedes aegypti
por: Runtuwene, Lucky R., et al.
Publicado: (2020)

NanoPipe—a web server for nanopore MinION sequencing data analysis
por: Shabardina, Victoria, et al.
Publicado: (2019)

Evolution of Multidrug Resistance in Plasmodium falciparum: a Longitudinal Study of Genetic Resistance Markers in the Greater Mekong Subregion
por: Imwong, Mallika, et al.
Publicado: (2021)

Drug resistance of Plasmodium falciparum and Plasmodium vivax isolates in Indonesia
por: Rahmasari, Farindira Vesti, et al.
Publicado: (2022)

Dengue transmission model by means of viremic adult immuno-competent mouse
por: Runtuwene, Lucky Ronald, et al.
Publicado: (2014)

Full-parasites: database of full-length cDNAs of apicomplexa parasites, 2010 update
por: Tuda, Josef, et al.
Publicado: (2011)

Impairment of Invasion and Maturation and Decreased Selectivity of Plasmodium falciparum in G6PD Viangchan and Mahidol Variants
por: Palasuwan, Duangdao, et al.
Publicado: (2021)

Elimination of Plasmodium falciparum in an area of multi-drug resistance
por: Lwin, Khin Maung, et al.
Publicado: (2015)

A Novel Diagnostic Method for Malaria Using Loop Mediated Isothermal Amplification (LAMP) and MinION Nanopore Sequencer
por: Imai, Kazuo, et al.
Publicado: (2017)

Protein profiling of mefloquine resistant Plasmodium falciparum using mass spectrometry-based proteomics
por: Reamtong, Onrapak, et al.
Publicado: (2015)

Prevalence of antifolate resistance mutations in Plasmodium falciparum isolates in Afghanistan
por: Awab, Ghulam R, et al.
Publicado: (2013)

Comparative analysis of targeted next-generation sequencing for Plasmodium falciparum drug resistance markers
por: Kunasol, Chanon, et al.
Publicado: (2022)

An outbreak of artemisinin resistant falciparum malaria in Eastern Thailand
por: Imwong, Mallika, et al.
Publicado: (2015)

K13 mutations and pfmdr1 copy number variation in Plasmodium falciparum malaria in Myanmar
por: Win, Aye A., et al.
Publicado: (2016)

Erratum to: K13 mutations and pfmdr1 copy number variation in Plasmodium falciparum malaria in Myanmar
por: Win, Aye A., et al.
Publicado: (2016)

Field diagnosis and genotyping of chikungunya virus using a dried reverse transcription loop-mediated isothermal amplification (LAMP) assay and MinION sequencing
por: Hayashida, Kyoko, et al.
Publicado: (2019)

Use of Blood Smears and Dried Blood Spots for Polymerase Chain Reaction–Based Detection and Quantification of Bacterial Infection and Plasmodium falciparum in Severely Ill Febrile African Children
por: Wihokhoen, Benchawan, et al.
Publicado: (2016)

Molecular characterization of Plasmodium falciparum antifolate resistance markers in Thailand between 2008 and 2016
por: Sugaram, Rungniran, et al.
Publicado: (2020)

A targeted approach with nanopore sequencing for the universal detection and identification of flaviviruses
por: Reteng, Patrick, et al.
Publicado: (2021)

Reduced Susceptibility of Plasmodium falciparum to Artesunate in Southern Myanmar
por: Kyaw, Myat P., et al.
Publicado: (2013)

No Evidence for Spread of Plasmodium falciparum Artemisinin Resistance to Savannakhet Province, Southern Laos
por: Mayxay, Mayfong, et al.
Publicado: (2012)

Intervals to Plasmodium falciparum recurrence after anti-malarial treatment in pregnancy: a longitudinal prospective cohort
por: Laochan, Natthapon, et al.
Publicado: (2015)

Application of loop-mediated isothermal amplification combined with lateral flow assay visualization of Plasmodium falciparum kelch 13 C580Y mutation for artemisinin resistance detection in clinical samples
por: Sanmoung, Wannida, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_osl2og8k2kepr6odf828nh9jup