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PCR-Stop analysis as a new tool for qPCR assay validation

Progressively more qPCR assays have been developed in recent years in numerous fields of application. These assays are routinely validated using calibration curves, but essential validation per se such as Poisson analysis is frequently neglected. However, validation is crucial for determination of r...

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Detalles Bibliográficos
Autores principales: Witte, Anna Kristina, Mester, Patrick, Fister, Susanne, Süß, Beate, Wagner, Martin, Rossmanith, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5974342/
https://www.ncbi.nlm.nih.gov/pubmed/29844518
http://dx.doi.org/10.1038/s41598-018-26116-x