Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks

Intense electric shocks of nanosecond (ns) duration can become a new modality for more efficient but safer defibrillation. We extended strength-duration curves for excitation of cardiomyocytes down to 200 ns, and compared electroporative damage by proportionally more intense shocks of different dura...

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Autores principales: Semenov, Iurii, Grigoryev, Sergey, Neuber, Johanna U., Zemlin, Christian W., Pakhomova, Olga N., Casciola, Maura, Pakhomov, Andrei G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5974370/
https://www.ncbi.nlm.nih.gov/pubmed/29844431
http://dx.doi.org/10.1038/s41598-018-26521-2
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author Semenov, Iurii
Grigoryev, Sergey
Neuber, Johanna U.
Zemlin, Christian W.
Pakhomova, Olga N.
Casciola, Maura
Pakhomov, Andrei G.
author_facet Semenov, Iurii
Grigoryev, Sergey
Neuber, Johanna U.
Zemlin, Christian W.
Pakhomova, Olga N.
Casciola, Maura
Pakhomov, Andrei G.
author_sort Semenov, Iurii
collection PubMed
description Intense electric shocks of nanosecond (ns) duration can become a new modality for more efficient but safer defibrillation. We extended strength-duration curves for excitation of cardiomyocytes down to 200 ns, and compared electroporative damage by proportionally more intense shocks of different duration. Enzymatically isolated murine, rabbit, and swine adult ventricular cardiomyocytes (VCM) were loaded with a Ca(2+) indicator Fluo-4 or Fluo-5N and subjected to shocks of increasing amplitude until a Ca(2+) transient was optically detected. Then, the voltage was increased 5-fold, and the electric cell injury was quantified by the uptake of a membrane permeability marker dye, propidium iodide. We established that: (1) Stimuli down to 200-ns duration can elicit Ca(2+) transients, although repeated ns shocks often evoke abnormal responses, (2) Stimulation thresholds expectedly increase as the shock duration decreases, similarly for VCMs from different species, (3) Stimulation threshold energy is minimal for the shortest shocks, (4) VCM orientation with respect to the electric field does not affect the threshold for ns shocks, and (5) The shortest shocks cause the least electroporation injury. These findings support further exploration of ns defibrillation, although abnormal response patterns to repetitive ns stimuli are of a concern and require mechanistic analysis.
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spelling pubmed-59743702018-05-31 Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks Semenov, Iurii Grigoryev, Sergey Neuber, Johanna U. Zemlin, Christian W. Pakhomova, Olga N. Casciola, Maura Pakhomov, Andrei G. Sci Rep Article Intense electric shocks of nanosecond (ns) duration can become a new modality for more efficient but safer defibrillation. We extended strength-duration curves for excitation of cardiomyocytes down to 200 ns, and compared electroporative damage by proportionally more intense shocks of different duration. Enzymatically isolated murine, rabbit, and swine adult ventricular cardiomyocytes (VCM) were loaded with a Ca(2+) indicator Fluo-4 or Fluo-5N and subjected to shocks of increasing amplitude until a Ca(2+) transient was optically detected. Then, the voltage was increased 5-fold, and the electric cell injury was quantified by the uptake of a membrane permeability marker dye, propidium iodide. We established that: (1) Stimuli down to 200-ns duration can elicit Ca(2+) transients, although repeated ns shocks often evoke abnormal responses, (2) Stimulation thresholds expectedly increase as the shock duration decreases, similarly for VCMs from different species, (3) Stimulation threshold energy is minimal for the shortest shocks, (4) VCM orientation with respect to the electric field does not affect the threshold for ns shocks, and (5) The shortest shocks cause the least electroporation injury. These findings support further exploration of ns defibrillation, although abnormal response patterns to repetitive ns stimuli are of a concern and require mechanistic analysis. Nature Publishing Group UK 2018-05-29 /pmc/articles/PMC5974370/ /pubmed/29844431 http://dx.doi.org/10.1038/s41598-018-26521-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Semenov, Iurii
Grigoryev, Sergey
Neuber, Johanna U.
Zemlin, Christian W.
Pakhomova, Olga N.
Casciola, Maura
Pakhomov, Andrei G.
Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks
title Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks
title_full Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks
title_fullStr Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks
title_full_unstemmed Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks
title_short Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks
title_sort excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5974370/
https://www.ncbi.nlm.nih.gov/pubmed/29844431
http://dx.doi.org/10.1038/s41598-018-26521-2
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