Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks
Intense electric shocks of nanosecond (ns) duration can become a new modality for more efficient but safer defibrillation. We extended strength-duration curves for excitation of cardiomyocytes down to 200 ns, and compared electroporative damage by proportionally more intense shocks of different dura...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5974370/ https://www.ncbi.nlm.nih.gov/pubmed/29844431 http://dx.doi.org/10.1038/s41598-018-26521-2 |
_version_ | 1783326805342552064 |
---|---|
author | Semenov, Iurii Grigoryev, Sergey Neuber, Johanna U. Zemlin, Christian W. Pakhomova, Olga N. Casciola, Maura Pakhomov, Andrei G. |
author_facet | Semenov, Iurii Grigoryev, Sergey Neuber, Johanna U. Zemlin, Christian W. Pakhomova, Olga N. Casciola, Maura Pakhomov, Andrei G. |
author_sort | Semenov, Iurii |
collection | PubMed |
description | Intense electric shocks of nanosecond (ns) duration can become a new modality for more efficient but safer defibrillation. We extended strength-duration curves for excitation of cardiomyocytes down to 200 ns, and compared electroporative damage by proportionally more intense shocks of different duration. Enzymatically isolated murine, rabbit, and swine adult ventricular cardiomyocytes (VCM) were loaded with a Ca(2+) indicator Fluo-4 or Fluo-5N and subjected to shocks of increasing amplitude until a Ca(2+) transient was optically detected. Then, the voltage was increased 5-fold, and the electric cell injury was quantified by the uptake of a membrane permeability marker dye, propidium iodide. We established that: (1) Stimuli down to 200-ns duration can elicit Ca(2+) transients, although repeated ns shocks often evoke abnormal responses, (2) Stimulation thresholds expectedly increase as the shock duration decreases, similarly for VCMs from different species, (3) Stimulation threshold energy is minimal for the shortest shocks, (4) VCM orientation with respect to the electric field does not affect the threshold for ns shocks, and (5) The shortest shocks cause the least electroporation injury. These findings support further exploration of ns defibrillation, although abnormal response patterns to repetitive ns stimuli are of a concern and require mechanistic analysis. |
format | Online Article Text |
id | pubmed-5974370 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59743702018-05-31 Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks Semenov, Iurii Grigoryev, Sergey Neuber, Johanna U. Zemlin, Christian W. Pakhomova, Olga N. Casciola, Maura Pakhomov, Andrei G. Sci Rep Article Intense electric shocks of nanosecond (ns) duration can become a new modality for more efficient but safer defibrillation. We extended strength-duration curves for excitation of cardiomyocytes down to 200 ns, and compared electroporative damage by proportionally more intense shocks of different duration. Enzymatically isolated murine, rabbit, and swine adult ventricular cardiomyocytes (VCM) were loaded with a Ca(2+) indicator Fluo-4 or Fluo-5N and subjected to shocks of increasing amplitude until a Ca(2+) transient was optically detected. Then, the voltage was increased 5-fold, and the electric cell injury was quantified by the uptake of a membrane permeability marker dye, propidium iodide. We established that: (1) Stimuli down to 200-ns duration can elicit Ca(2+) transients, although repeated ns shocks often evoke abnormal responses, (2) Stimulation thresholds expectedly increase as the shock duration decreases, similarly for VCMs from different species, (3) Stimulation threshold energy is minimal for the shortest shocks, (4) VCM orientation with respect to the electric field does not affect the threshold for ns shocks, and (5) The shortest shocks cause the least electroporation injury. These findings support further exploration of ns defibrillation, although abnormal response patterns to repetitive ns stimuli are of a concern and require mechanistic analysis. Nature Publishing Group UK 2018-05-29 /pmc/articles/PMC5974370/ /pubmed/29844431 http://dx.doi.org/10.1038/s41598-018-26521-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Semenov, Iurii Grigoryev, Sergey Neuber, Johanna U. Zemlin, Christian W. Pakhomova, Olga N. Casciola, Maura Pakhomov, Andrei G. Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks |
title | Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks |
title_full | Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks |
title_fullStr | Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks |
title_full_unstemmed | Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks |
title_short | Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks |
title_sort | excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5974370/ https://www.ncbi.nlm.nih.gov/pubmed/29844431 http://dx.doi.org/10.1038/s41598-018-26521-2 |
work_keys_str_mv | AT semenoviurii excitationandinjuryofadultventricularcardiomyocytesbynanotomillisecondelectricshocks AT grigoryevsergey excitationandinjuryofadultventricularcardiomyocytesbynanotomillisecondelectricshocks AT neuberjohannau excitationandinjuryofadultventricularcardiomyocytesbynanotomillisecondelectricshocks AT zemlinchristianw excitationandinjuryofadultventricularcardiomyocytesbynanotomillisecondelectricshocks AT pakhomovaolgan excitationandinjuryofadultventricularcardiomyocytesbynanotomillisecondelectricshocks AT casciolamaura excitationandinjuryofadultventricularcardiomyocytesbynanotomillisecondelectricshocks AT pakhomovandreig excitationandinjuryofadultventricularcardiomyocytesbynanotomillisecondelectricshocks |