Cargando…

Investigation of dose-rate effects and cell-cycle distribution under protracted exposure to ionizing radiation for various dose-rates

During exposure to ionizing radiation, sub-lethal damage repair (SLDR) competes with DNA damage induction in cultured cells. By virtue of SLDR, cell survival increases with decrease of dose-rate, so-called dose-rate effects (DREs). Here, we focused on a wide dose-rate range and investigated the chan...

Descripción completa

Detalles Bibliográficos
Autores principales: Matsuya, Yusuke, McMahon, Stephen J., Tsutsumi, Kaori, Sasaki, Kohei, Okuyama, Go, Yoshii, Yuji, Mori, Ryosuke, Oikawa, Joma, Prise, Kevin M., Date, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5974424/
https://www.ncbi.nlm.nih.gov/pubmed/29844494
http://dx.doi.org/10.1038/s41598-018-26556-5
_version_ 1783326816830750720
author Matsuya, Yusuke
McMahon, Stephen J.
Tsutsumi, Kaori
Sasaki, Kohei
Okuyama, Go
Yoshii, Yuji
Mori, Ryosuke
Oikawa, Joma
Prise, Kevin M.
Date, Hiroyuki
author_facet Matsuya, Yusuke
McMahon, Stephen J.
Tsutsumi, Kaori
Sasaki, Kohei
Okuyama, Go
Yoshii, Yuji
Mori, Ryosuke
Oikawa, Joma
Prise, Kevin M.
Date, Hiroyuki
author_sort Matsuya, Yusuke
collection PubMed
description During exposure to ionizing radiation, sub-lethal damage repair (SLDR) competes with DNA damage induction in cultured cells. By virtue of SLDR, cell survival increases with decrease of dose-rate, so-called dose-rate effects (DREs). Here, we focused on a wide dose-rate range and investigated the change of cell-cycle distribution during X-ray protracted exposure and dose-response curves via hybrid analysis with a combination of in vitro experiments and mathematical modelling. In the course of flow-cytometric cell-cycle analysis and clonogenic assays, we found the following responses in CHO-K1 cells: (1) The fraction of cells in S phase gradually increases during 6 h exposure at 3.0 Gy/h, which leads to radio-resistance. (2) Slight cell accumulation in S and G(2)/M phases is observed after exposure at 6.0 Gy/h for more than 10 hours. This suggests that an increase of SLDR rate for cells in S phase during irradiation may be a reproducible factor to describe changes in the dose-response curve at dose-rates of 3.0 and 6.0 Gy/h. By re-evaluating cell survival for various dose-rates of 0.186–60.0 Gy/h considering experimental-based DNA content and SLDR, it is suggested that the change of S phase fraction during irradiation modulates the dose-response curve and is possibly responsible for some inverse DREs.
format Online
Article
Text
id pubmed-5974424
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-59744242018-05-31 Investigation of dose-rate effects and cell-cycle distribution under protracted exposure to ionizing radiation for various dose-rates Matsuya, Yusuke McMahon, Stephen J. Tsutsumi, Kaori Sasaki, Kohei Okuyama, Go Yoshii, Yuji Mori, Ryosuke Oikawa, Joma Prise, Kevin M. Date, Hiroyuki Sci Rep Article During exposure to ionizing radiation, sub-lethal damage repair (SLDR) competes with DNA damage induction in cultured cells. By virtue of SLDR, cell survival increases with decrease of dose-rate, so-called dose-rate effects (DREs). Here, we focused on a wide dose-rate range and investigated the change of cell-cycle distribution during X-ray protracted exposure and dose-response curves via hybrid analysis with a combination of in vitro experiments and mathematical modelling. In the course of flow-cytometric cell-cycle analysis and clonogenic assays, we found the following responses in CHO-K1 cells: (1) The fraction of cells in S phase gradually increases during 6 h exposure at 3.0 Gy/h, which leads to radio-resistance. (2) Slight cell accumulation in S and G(2)/M phases is observed after exposure at 6.0 Gy/h for more than 10 hours. This suggests that an increase of SLDR rate for cells in S phase during irradiation may be a reproducible factor to describe changes in the dose-response curve at dose-rates of 3.0 and 6.0 Gy/h. By re-evaluating cell survival for various dose-rates of 0.186–60.0 Gy/h considering experimental-based DNA content and SLDR, it is suggested that the change of S phase fraction during irradiation modulates the dose-response curve and is possibly responsible for some inverse DREs. Nature Publishing Group UK 2018-05-29 /pmc/articles/PMC5974424/ /pubmed/29844494 http://dx.doi.org/10.1038/s41598-018-26556-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Matsuya, Yusuke
McMahon, Stephen J.
Tsutsumi, Kaori
Sasaki, Kohei
Okuyama, Go
Yoshii, Yuji
Mori, Ryosuke
Oikawa, Joma
Prise, Kevin M.
Date, Hiroyuki
Investigation of dose-rate effects and cell-cycle distribution under protracted exposure to ionizing radiation for various dose-rates
title Investigation of dose-rate effects and cell-cycle distribution under protracted exposure to ionizing radiation for various dose-rates
title_full Investigation of dose-rate effects and cell-cycle distribution under protracted exposure to ionizing radiation for various dose-rates
title_fullStr Investigation of dose-rate effects and cell-cycle distribution under protracted exposure to ionizing radiation for various dose-rates
title_full_unstemmed Investigation of dose-rate effects and cell-cycle distribution under protracted exposure to ionizing radiation for various dose-rates
title_short Investigation of dose-rate effects and cell-cycle distribution under protracted exposure to ionizing radiation for various dose-rates
title_sort investigation of dose-rate effects and cell-cycle distribution under protracted exposure to ionizing radiation for various dose-rates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5974424/
https://www.ncbi.nlm.nih.gov/pubmed/29844494
http://dx.doi.org/10.1038/s41598-018-26556-5
work_keys_str_mv AT matsuyayusuke investigationofdoserateeffectsandcellcycledistributionunderprotractedexposuretoionizingradiationforvariousdoserates
AT mcmahonstephenj investigationofdoserateeffectsandcellcycledistributionunderprotractedexposuretoionizingradiationforvariousdoserates
AT tsutsumikaori investigationofdoserateeffectsandcellcycledistributionunderprotractedexposuretoionizingradiationforvariousdoserates
AT sasakikohei investigationofdoserateeffectsandcellcycledistributionunderprotractedexposuretoionizingradiationforvariousdoserates
AT okuyamago investigationofdoserateeffectsandcellcycledistributionunderprotractedexposuretoionizingradiationforvariousdoserates
AT yoshiiyuji investigationofdoserateeffectsandcellcycledistributionunderprotractedexposuretoionizingradiationforvariousdoserates
AT moriryosuke investigationofdoserateeffectsandcellcycledistributionunderprotractedexposuretoionizingradiationforvariousdoserates
AT oikawajoma investigationofdoserateeffectsandcellcycledistributionunderprotractedexposuretoionizingradiationforvariousdoserates
AT prisekevinm investigationofdoserateeffectsandcellcycledistributionunderprotractedexposuretoionizingradiationforvariousdoserates
AT datehiroyuki investigationofdoserateeffectsandcellcycledistributionunderprotractedexposuretoionizingradiationforvariousdoserates