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Rapid production of pure recombinant actin isoforms in Pichia pastoris

Actins are major eukaryotic cytoskeletal proteins, and they are involved in many important cell functions, including cell division, cell polarity, wound healing and muscle contraction. Despite obvious drawbacks, muscle actin, which is easily purified, is used extensively for biochemical studies of t...

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Detalles Bibliográficos
Autores principales: Hatano, Tomoyuki, Alioto, Salvatore, Roscioli, Emanuele, Palani, Saravanan, Clarke, Scott T., Kamnev, Anton, Hernandez-Fernaud, Juan Ramon, Sivashanmugam, Lavanya, Chapa-y-Lazo, Bernardo, Jones, Alexandra M. E., Robinson, Robert C., Sampath, Karuna, Mishima, Masanori, McAinsh, Andrew D., Goode, Bruce L., Balasubramanian, Mohan K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5976186/
https://www.ncbi.nlm.nih.gov/pubmed/29535210
http://dx.doi.org/10.1242/jcs.213827