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Improved sensitivity for detection of breast cancer by combination of miR-34a and tumor markers CA 15-3 or CEA

BACKGROUND: MicroRNAs biomarkers have shown value for diagnosis and prognosis of various cancers. Combination with established tumor markers has rarely been done. RESULTS: Breast cancer patients had significantly higher serum RNA loads (AUC 0.665), lower miR-34a (AUC 0.772), higher CEA and CA 15-3 l...

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Autores principales: Zaleski, Martin, Kobilay, Makbule, Schroeder, Lars, Debald, Manuel, Semaan, Alexander, Hettwer, Karina, Uhlig, Steffen, Kuhn, Walther, Hartmann, Gunther, Holdenrieder, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5976482/
https://www.ncbi.nlm.nih.gov/pubmed/29854296
http://dx.doi.org/10.18632/oncotarget.25077
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author Zaleski, Martin
Kobilay, Makbule
Schroeder, Lars
Debald, Manuel
Semaan, Alexander
Hettwer, Karina
Uhlig, Steffen
Kuhn, Walther
Hartmann, Gunther
Holdenrieder, Stefan
author_facet Zaleski, Martin
Kobilay, Makbule
Schroeder, Lars
Debald, Manuel
Semaan, Alexander
Hettwer, Karina
Uhlig, Steffen
Kuhn, Walther
Hartmann, Gunther
Holdenrieder, Stefan
author_sort Zaleski, Martin
collection PubMed
description BACKGROUND: MicroRNAs biomarkers have shown value for diagnosis and prognosis of various cancers. Combination with established tumor markers has rarely been done. RESULTS: Breast cancer patients had significantly higher serum RNA loads (AUC 0.665), lower miR-34a (AUC 0.772), higher CEA and CA 15-3 levels (AUCs 0.717 and 0.721) than healthy controls. miR-34a correlated with tumor stage and hormone receptor status. There was no significant difference between groups for all other miRNAs. Combination of miR-34a with CEA or CA 15-3 led to improved AUCs of 0.844 and 0.800, respectively. Sensitivity of miR-34a and CA 15-3 reached 56.1% at 95% specificity. When compared with benign breast diseases, combination of miR-34a (AUC 0.719) and CEA (0.623) or CA 15-3 (0.619) resulted in improved performances (0.794 and 0.741). Sensitivity of miR-34a and CA 15-3 reached 53.7% at 95% specificity. CONCLUSION: While miR-34a provides valuable information for diagnosis and staging, combination with tumor markers CA15-3 or CEA improves the sensitivity for breast cancer detection. PATIENTS AND METHODS: The diagnostic relevance of the miR-21, miR-34a, miR-92a, miR-155, miR-222 and miR-let-7c was tested in sera of 103 individuals (55 breast cancer, 20 benign breast diseases, 28 healthy controls). MiRNAs were detected by quantitative rt-PCR after extraction and reverse transcription. Cel-miR-39 and miR-16 were used for normalization. Established tumor markers CEA, CA 15-3, CA 19-9 and CA 125 were measured by automatized immunoassays. Diagnostic performance was tested by areas under the curve (AUC) of receiver operating characteristic (ROC) curves and sensitivities at 90% and 95% specificity.
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spelling pubmed-59764822018-05-31 Improved sensitivity for detection of breast cancer by combination of miR-34a and tumor markers CA 15-3 or CEA Zaleski, Martin Kobilay, Makbule Schroeder, Lars Debald, Manuel Semaan, Alexander Hettwer, Karina Uhlig, Steffen Kuhn, Walther Hartmann, Gunther Holdenrieder, Stefan Oncotarget Research Paper BACKGROUND: MicroRNAs biomarkers have shown value for diagnosis and prognosis of various cancers. Combination with established tumor markers has rarely been done. RESULTS: Breast cancer patients had significantly higher serum RNA loads (AUC 0.665), lower miR-34a (AUC 0.772), higher CEA and CA 15-3 levels (AUCs 0.717 and 0.721) than healthy controls. miR-34a correlated with tumor stage and hormone receptor status. There was no significant difference between groups for all other miRNAs. Combination of miR-34a with CEA or CA 15-3 led to improved AUCs of 0.844 and 0.800, respectively. Sensitivity of miR-34a and CA 15-3 reached 56.1% at 95% specificity. When compared with benign breast diseases, combination of miR-34a (AUC 0.719) and CEA (0.623) or CA 15-3 (0.619) resulted in improved performances (0.794 and 0.741). Sensitivity of miR-34a and CA 15-3 reached 53.7% at 95% specificity. CONCLUSION: While miR-34a provides valuable information for diagnosis and staging, combination with tumor markers CA15-3 or CEA improves the sensitivity for breast cancer detection. PATIENTS AND METHODS: The diagnostic relevance of the miR-21, miR-34a, miR-92a, miR-155, miR-222 and miR-let-7c was tested in sera of 103 individuals (55 breast cancer, 20 benign breast diseases, 28 healthy controls). MiRNAs were detected by quantitative rt-PCR after extraction and reverse transcription. Cel-miR-39 and miR-16 were used for normalization. Established tumor markers CEA, CA 15-3, CA 19-9 and CA 125 were measured by automatized immunoassays. Diagnostic performance was tested by areas under the curve (AUC) of receiver operating characteristic (ROC) curves and sensitivities at 90% and 95% specificity. Impact Journals LLC 2018-04-27 /pmc/articles/PMC5976482/ /pubmed/29854296 http://dx.doi.org/10.18632/oncotarget.25077 Text en Copyright: © 2018 Zaleski et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Zaleski, Martin
Kobilay, Makbule
Schroeder, Lars
Debald, Manuel
Semaan, Alexander
Hettwer, Karina
Uhlig, Steffen
Kuhn, Walther
Hartmann, Gunther
Holdenrieder, Stefan
Improved sensitivity for detection of breast cancer by combination of miR-34a and tumor markers CA 15-3 or CEA
title Improved sensitivity for detection of breast cancer by combination of miR-34a and tumor markers CA 15-3 or CEA
title_full Improved sensitivity for detection of breast cancer by combination of miR-34a and tumor markers CA 15-3 or CEA
title_fullStr Improved sensitivity for detection of breast cancer by combination of miR-34a and tumor markers CA 15-3 or CEA
title_full_unstemmed Improved sensitivity for detection of breast cancer by combination of miR-34a and tumor markers CA 15-3 or CEA
title_short Improved sensitivity for detection of breast cancer by combination of miR-34a and tumor markers CA 15-3 or CEA
title_sort improved sensitivity for detection of breast cancer by combination of mir-34a and tumor markers ca 15-3 or cea
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5976482/
https://www.ncbi.nlm.nih.gov/pubmed/29854296
http://dx.doi.org/10.18632/oncotarget.25077
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