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Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort
This study is focused on the prevalent NS5 coding region resistance-associated substitutions (RASs) in DAA-naive genotype (GT)1 HCV-infected patients and their potential impact on success rates. Plasma RNA from 81 GT1 HCV-infected patients was extracted prior to an in-house nested RT-PCR of the NS5...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977216/ https://www.ncbi.nlm.nih.gov/pubmed/29701642 http://dx.doi.org/10.3390/v10050223 |
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author | Brandão, Ruben Marcelino, Rute Gonçalves, Fátima Diogo, Isabel Carvalho, Ana Cabanas, Joaquim Costa, Inês Brogueira, Pedro Ventura, Fernando Miranda, Ana Mansinho, Kamal Gomes, Perpétua |
author_facet | Brandão, Ruben Marcelino, Rute Gonçalves, Fátima Diogo, Isabel Carvalho, Ana Cabanas, Joaquim Costa, Inês Brogueira, Pedro Ventura, Fernando Miranda, Ana Mansinho, Kamal Gomes, Perpétua |
author_sort | Brandão, Ruben |
collection | PubMed |
description | This study is focused on the prevalent NS5 coding region resistance-associated substitutions (RASs) in DAA-naive genotype (GT)1 HCV-infected patients and their potential impact on success rates. Plasma RNA from 81 GT1 HCV-infected patients was extracted prior to an in-house nested RT-PCR of the NS5 coding region, which is followed by Sanger population sequencing. NS5A RASs were present in 28.4% (23/81) of all GT1-infected patients with 9.9% (8/81) having the Y93C/H mutation. NS5B RASs showed a prevalence of 14.8% (12/81) and were only detected in GT1b. Overall 38.3% (31/81) of all GT1 HCV-infected patients presented baseline RASs. The obtained data supports the usefulness of resistance testing prior to treatment since a statistically significant association was found between treatment failure and the baseline presence of specific NS5 RASs known as Y93C/H (p = 0.04). |
format | Online Article Text |
id | pubmed-5977216 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-59772162018-06-01 Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort Brandão, Ruben Marcelino, Rute Gonçalves, Fátima Diogo, Isabel Carvalho, Ana Cabanas, Joaquim Costa, Inês Brogueira, Pedro Ventura, Fernando Miranda, Ana Mansinho, Kamal Gomes, Perpétua Viruses Article This study is focused on the prevalent NS5 coding region resistance-associated substitutions (RASs) in DAA-naive genotype (GT)1 HCV-infected patients and their potential impact on success rates. Plasma RNA from 81 GT1 HCV-infected patients was extracted prior to an in-house nested RT-PCR of the NS5 coding region, which is followed by Sanger population sequencing. NS5A RASs were present in 28.4% (23/81) of all GT1-infected patients with 9.9% (8/81) having the Y93C/H mutation. NS5B RASs showed a prevalence of 14.8% (12/81) and were only detected in GT1b. Overall 38.3% (31/81) of all GT1 HCV-infected patients presented baseline RASs. The obtained data supports the usefulness of resistance testing prior to treatment since a statistically significant association was found between treatment failure and the baseline presence of specific NS5 RASs known as Y93C/H (p = 0.04). MDPI 2018-04-26 /pmc/articles/PMC5977216/ /pubmed/29701642 http://dx.doi.org/10.3390/v10050223 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Brandão, Ruben Marcelino, Rute Gonçalves, Fátima Diogo, Isabel Carvalho, Ana Cabanas, Joaquim Costa, Inês Brogueira, Pedro Ventura, Fernando Miranda, Ana Mansinho, Kamal Gomes, Perpétua Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort |
title | Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort |
title_full | Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort |
title_fullStr | Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort |
title_full_unstemmed | Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort |
title_short | Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort |
title_sort | characterization of ns5a and ns5b resistance-associated substitutions from genotype 1 hepatitis c virus infected patients in a portuguese cohort |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977216/ https://www.ncbi.nlm.nih.gov/pubmed/29701642 http://dx.doi.org/10.3390/v10050223 |
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