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Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort

This study is focused on the prevalent NS5 coding region resistance-associated substitutions (RASs) in DAA-naive genotype (GT)1 HCV-infected patients and their potential impact on success rates. Plasma RNA from 81 GT1 HCV-infected patients was extracted prior to an in-house nested RT-PCR of the NS5...

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Autores principales: Brandão, Ruben, Marcelino, Rute, Gonçalves, Fátima, Diogo, Isabel, Carvalho, Ana, Cabanas, Joaquim, Costa, Inês, Brogueira, Pedro, Ventura, Fernando, Miranda, Ana, Mansinho, Kamal, Gomes, Perpétua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977216/
https://www.ncbi.nlm.nih.gov/pubmed/29701642
http://dx.doi.org/10.3390/v10050223
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author Brandão, Ruben
Marcelino, Rute
Gonçalves, Fátima
Diogo, Isabel
Carvalho, Ana
Cabanas, Joaquim
Costa, Inês
Brogueira, Pedro
Ventura, Fernando
Miranda, Ana
Mansinho, Kamal
Gomes, Perpétua
author_facet Brandão, Ruben
Marcelino, Rute
Gonçalves, Fátima
Diogo, Isabel
Carvalho, Ana
Cabanas, Joaquim
Costa, Inês
Brogueira, Pedro
Ventura, Fernando
Miranda, Ana
Mansinho, Kamal
Gomes, Perpétua
author_sort Brandão, Ruben
collection PubMed
description This study is focused on the prevalent NS5 coding region resistance-associated substitutions (RASs) in DAA-naive genotype (GT)1 HCV-infected patients and their potential impact on success rates. Plasma RNA from 81 GT1 HCV-infected patients was extracted prior to an in-house nested RT-PCR of the NS5 coding region, which is followed by Sanger population sequencing. NS5A RASs were present in 28.4% (23/81) of all GT1-infected patients with 9.9% (8/81) having the Y93C/H mutation. NS5B RASs showed a prevalence of 14.8% (12/81) and were only detected in GT1b. Overall 38.3% (31/81) of all GT1 HCV-infected patients presented baseline RASs. The obtained data supports the usefulness of resistance testing prior to treatment since a statistically significant association was found between treatment failure and the baseline presence of specific NS5 RASs known as Y93C/H (p = 0.04).
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spelling pubmed-59772162018-06-01 Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort Brandão, Ruben Marcelino, Rute Gonçalves, Fátima Diogo, Isabel Carvalho, Ana Cabanas, Joaquim Costa, Inês Brogueira, Pedro Ventura, Fernando Miranda, Ana Mansinho, Kamal Gomes, Perpétua Viruses Article This study is focused on the prevalent NS5 coding region resistance-associated substitutions (RASs) in DAA-naive genotype (GT)1 HCV-infected patients and their potential impact on success rates. Plasma RNA from 81 GT1 HCV-infected patients was extracted prior to an in-house nested RT-PCR of the NS5 coding region, which is followed by Sanger population sequencing. NS5A RASs were present in 28.4% (23/81) of all GT1-infected patients with 9.9% (8/81) having the Y93C/H mutation. NS5B RASs showed a prevalence of 14.8% (12/81) and were only detected in GT1b. Overall 38.3% (31/81) of all GT1 HCV-infected patients presented baseline RASs. The obtained data supports the usefulness of resistance testing prior to treatment since a statistically significant association was found between treatment failure and the baseline presence of specific NS5 RASs known as Y93C/H (p = 0.04). MDPI 2018-04-26 /pmc/articles/PMC5977216/ /pubmed/29701642 http://dx.doi.org/10.3390/v10050223 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Brandão, Ruben
Marcelino, Rute
Gonçalves, Fátima
Diogo, Isabel
Carvalho, Ana
Cabanas, Joaquim
Costa, Inês
Brogueira, Pedro
Ventura, Fernando
Miranda, Ana
Mansinho, Kamal
Gomes, Perpétua
Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort
title Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort
title_full Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort
title_fullStr Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort
title_full_unstemmed Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort
title_short Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort
title_sort characterization of ns5a and ns5b resistance-associated substitutions from genotype 1 hepatitis c virus infected patients in a portuguese cohort
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977216/
https://www.ncbi.nlm.nih.gov/pubmed/29701642
http://dx.doi.org/10.3390/v10050223
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