Potential high-frequency off-target mutagenesis induced by CRISPR/Cas9 in Arabidopsis and its prevention

KEY MESSAGE: We present novel observations of high-specificity SpCas9 variants, sgRNA expression strategies based on mutant sgRNA scaffold and tRNA processing system, and CRISPR/Cas9-mediated T-DNA integrations. ABSTRACT: Specificity of CRISPR/Cas9 tools has been a major concern along with the repor...

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Detalles Bibliográficos
Autores principales: Zhang, Qiang, Xing, Hui-Li, Wang, Zhi-Ping, Zhang, Hai-Yan, Yang, Fang, Wang, Xue-Chen, Chen, Qi-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5978904/
https://www.ncbi.nlm.nih.gov/pubmed/29476306
http://dx.doi.org/10.1007/s11103-018-0709-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5978904/
https://www.ncbi.nlm.nih.gov/pubmed/29476306
http://dx.doi.org/10.1007/s11103-018-0709-x

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