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Potential high-frequency off-target mutagenesis induced by CRISPR/Cas9 in Arabidopsis and its prevention

KEY MESSAGE: We present novel observations of high-specificity SpCas9 variants, sgRNA expression strategies based on mutant sgRNA scaffold and tRNA processing system, and CRISPR/Cas9-mediated T-DNA integrations. ABSTRACT: Specificity of CRISPR/Cas9 tools has been a major concern along with the repor...

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Detalles Bibliográficos
Autores principales:	Zhang, Qiang, Xing, Hui-Li, Wang, Zhi-Ping, Zhang, Hai-Yan, Yang, Fang, Wang, Xue-Chen, Chen, Qi-Jun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Netherlands 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5978904/ https://www.ncbi.nlm.nih.gov/pubmed/29476306 http://dx.doi.org/10.1007/s11103-018-0709-x

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