Influence of the fixation/permeabilization step on peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria

Fluorescence in situ Hybridization (FISH) is a versatile, widespread and widely- used technique in microbiology. The first step of FISH—fixation/permeabilization—is crucial to the outcome of the method. This work aimed to systematically evaluate fixation/permeabilization protocols employing ethanol,...

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Detalles Bibliográficos
Autores principales: Rocha, Rui, Almeida, Carina, Azevedo, Nuno F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979007/
https://www.ncbi.nlm.nih.gov/pubmed/29851961
http://dx.doi.org/10.1371/journal.pone.0196522

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979007/
https://www.ncbi.nlm.nih.gov/pubmed/29851961
http://dx.doi.org/10.1371/journal.pone.0196522

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