The Combinational Use of CRISPR/Cas9 and Targeted Toxin Technology Enables Efficient Isolation of Bi-Allelic Knockout Non-Human Mammalian Clones

Recent advances in genome editing systems such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein-9 nuclease (CRISPR/Cas9) have facilitated genomic modification in mammalian cells. However, most systems employ transient treatment with selective drugs such as purom...

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Detalles Bibliográficos
Autores principales: Watanabe, Satoshi, Sakurai, Takayuki, Nakamura, Shingo, Miyoshi, Kazuchika, Sato, Masahiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979347/
https://www.ncbi.nlm.nih.gov/pubmed/29617297
http://dx.doi.org/10.3390/ijms19041075

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979347/
https://www.ncbi.nlm.nih.gov/pubmed/29617297
http://dx.doi.org/10.3390/ijms19041075

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