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Orthogonality of Pyrrolysine tRNA in the Xenopus oocyte

Chemical aminoacylation of orthogonal tRNA allows for the genetic encoding of a wide range of synthetic amino acids without the need to evolve specific aminoacyl-tRNA synthetases. This method, when paired with protein expression in the Xenopus laevis oocyte expression system, can extract atomic scal...

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Detalles Bibliográficos
Autores principales: Infield, Daniel T., Lueck, John D., Galpin, Jason D., Galles, Grace D., Ahern, Christopher A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5980078/
https://www.ncbi.nlm.nih.gov/pubmed/29581437
http://dx.doi.org/10.1038/s41598-018-23201-z