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Orthogonality of Pyrrolysine tRNA in the Xenopus oocyte
Chemical aminoacylation of orthogonal tRNA allows for the genetic encoding of a wide range of synthetic amino acids without the need to evolve specific aminoacyl-tRNA synthetases. This method, when paired with protein expression in the Xenopus laevis oocyte expression system, can extract atomic scal...
Autores principales: | Infield, Daniel T., Lueck, John D., Galpin, Jason D., Galles, Grace D., Ahern, Christopher A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5980078/ https://www.ncbi.nlm.nih.gov/pubmed/29581437 http://dx.doi.org/10.1038/s41598-018-23201-z |
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