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CRISPR/Cas9 mediated targeting of multiple genes in Dictyostelium

CRISPR/Cas9 has emerged in various organisms as a powerful technology for targeted gene knockout; however, no reports of editing the Dictyostelium genome efficiently using this system are available. We describe here the application of CRISPR/Cas9-mediated gene modification in Dictyostelium. The endo...

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Detalles Bibliográficos
Autores principales: Sekine, Ryoya, Kawata, Takefumi, Muramoto, Tetsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5981456/
https://www.ncbi.nlm.nih.gov/pubmed/29855514
http://dx.doi.org/10.1038/s41598-018-26756-z