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CRISPR/Cas9 mediated targeting of multiple genes in Dictyostelium
CRISPR/Cas9 has emerged in various organisms as a powerful technology for targeted gene knockout; however, no reports of editing the Dictyostelium genome efficiently using this system are available. We describe here the application of CRISPR/Cas9-mediated gene modification in Dictyostelium. The endo...
Autores principales: | Sekine, Ryoya, Kawata, Takefumi, Muramoto, Tetsuya |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5981456/ https://www.ncbi.nlm.nih.gov/pubmed/29855514 http://dx.doi.org/10.1038/s41598-018-26756-z |
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