A Cloning-Free Method for CRISPR/Cas9-Mediated Genome Editing in Fission Yeast

The CRISPR/Cas9 system, which relies on RNA‐guided DNA cleavage to induce site-specific DNA double-strand breaks, is a powerful tool for genome editing. This system has been successfully adapted for the fission yeast Schizosaccharomyces pombe by expressing Cas9 and the single-guide RNA (sgRNA) from...

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Detalles Bibliográficos
Autores principales: Zhang, Xiao-Ran, He, Jia-Bei, Wang, Yi-Zheng, Du, Li-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2018
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5982833/
https://www.ncbi.nlm.nih.gov/pubmed/29703785
http://dx.doi.org/10.1534/g3.118.200164

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5982833/
https://www.ncbi.nlm.nih.gov/pubmed/29703785
http://dx.doi.org/10.1534/g3.118.200164

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