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Use of trachea-bronchial swab qPCR testing to confirm Mycoplasma hyopneumoniae seropositivity in an SPF breeding herd

BACKGROUND: A dedicated program to monitor for freedom of several economically important diseases is present within most of the breeding companies that currently deliver high health breeding animals to their customers. Serology is therefore the preferential approach in order to screen for most of th...

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Autores principales: Vangroenweghe, Frédéric, Willems, Eveline, Malášek, Jiří, Thas, Olivier, Maes, Dominiek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5984420/
https://www.ncbi.nlm.nih.gov/pubmed/29881637
http://dx.doi.org/10.1186/s40813-018-0088-3
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author Vangroenweghe, Frédéric
Willems, Eveline
Malášek, Jiří
Thas, Olivier
Maes, Dominiek
author_facet Vangroenweghe, Frédéric
Willems, Eveline
Malášek, Jiří
Thas, Olivier
Maes, Dominiek
author_sort Vangroenweghe, Frédéric
collection PubMed
description BACKGROUND: A dedicated program to monitor for freedom of several economically important diseases is present within most of the breeding companies that currently deliver high health breeding animals to their customers. Serology is therefore the preferential approach in order to screen for most of these diseases, including Mycoplasma hyopneumoniae (M. hyopneumoniae). However, in case of positive serology, further decisions on farm health status and the related consequences should be based on additional confirmation tests. CASE PRESENTATION: The current case report demonstrates that tracheo-bronchial swab (TBS) sampling is a suitable alternative to confirm a suspect M. hyopneumoniae-seropositive situation. A Central-European SPF herd was shown positive (90% positive, 10% suspect; n = 10) for M. hyopneumoniae using the conventional ELISA serology (Idexx HerdChek Mhyo ELISA) and a second ELISA test (IDEIA™ Mycoplasma hyopneumoniae EIA kit) did not exclude potential M. hyopneumoniae infection (10% positive, 70% suspect; n = 10). Further follow-up remained inconclusive on both tests. Throughout the entire monitoring period of 6 months, no coughing, necropsy lesions or lesions at slaughter could be detected which could confirm the M. hyopneumoniae health status. TBS sampling was used to confirm the health status for M. hyopneumoniae. In total, 162 samples were collected at different ages (n = 18 per age category): piglets at 3–6–9-12 and 15 wks of age, rearing gilts at 18–21-24 and 27 weeks of age. Collected TBS samples were negative for M. hyopneumoniae until 15 wks of age, but rearing gilts were highly M. hyopneumoniae-positive from 18 wks onwards with 87–100% M. hyopneumoniae-positive animals and PCR Ct-values between 25 and 33. CONCLUSIONS: This case report shows that collection of TBS samples to confirm the M. hyopneumoniae infection status of a breeding herd was able to provide additional information to serology in order to make crucial decisions concerning health management and eradication strategies within the breeding herd.
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spelling pubmed-59844202018-06-07 Use of trachea-bronchial swab qPCR testing to confirm Mycoplasma hyopneumoniae seropositivity in an SPF breeding herd Vangroenweghe, Frédéric Willems, Eveline Malášek, Jiří Thas, Olivier Maes, Dominiek Porcine Health Manag Case Report BACKGROUND: A dedicated program to monitor for freedom of several economically important diseases is present within most of the breeding companies that currently deliver high health breeding animals to their customers. Serology is therefore the preferential approach in order to screen for most of these diseases, including Mycoplasma hyopneumoniae (M. hyopneumoniae). However, in case of positive serology, further decisions on farm health status and the related consequences should be based on additional confirmation tests. CASE PRESENTATION: The current case report demonstrates that tracheo-bronchial swab (TBS) sampling is a suitable alternative to confirm a suspect M. hyopneumoniae-seropositive situation. A Central-European SPF herd was shown positive (90% positive, 10% suspect; n = 10) for M. hyopneumoniae using the conventional ELISA serology (Idexx HerdChek Mhyo ELISA) and a second ELISA test (IDEIA™ Mycoplasma hyopneumoniae EIA kit) did not exclude potential M. hyopneumoniae infection (10% positive, 70% suspect; n = 10). Further follow-up remained inconclusive on both tests. Throughout the entire monitoring period of 6 months, no coughing, necropsy lesions or lesions at slaughter could be detected which could confirm the M. hyopneumoniae health status. TBS sampling was used to confirm the health status for M. hyopneumoniae. In total, 162 samples were collected at different ages (n = 18 per age category): piglets at 3–6–9-12 and 15 wks of age, rearing gilts at 18–21-24 and 27 weeks of age. Collected TBS samples were negative for M. hyopneumoniae until 15 wks of age, but rearing gilts were highly M. hyopneumoniae-positive from 18 wks onwards with 87–100% M. hyopneumoniae-positive animals and PCR Ct-values between 25 and 33. CONCLUSIONS: This case report shows that collection of TBS samples to confirm the M. hyopneumoniae infection status of a breeding herd was able to provide additional information to serology in order to make crucial decisions concerning health management and eradication strategies within the breeding herd. BioMed Central 2018-06-01 /pmc/articles/PMC5984420/ /pubmed/29881637 http://dx.doi.org/10.1186/s40813-018-0088-3 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Case Report
Vangroenweghe, Frédéric
Willems, Eveline
Malášek, Jiří
Thas, Olivier
Maes, Dominiek
Use of trachea-bronchial swab qPCR testing to confirm Mycoplasma hyopneumoniae seropositivity in an SPF breeding herd
title Use of trachea-bronchial swab qPCR testing to confirm Mycoplasma hyopneumoniae seropositivity in an SPF breeding herd
title_full Use of trachea-bronchial swab qPCR testing to confirm Mycoplasma hyopneumoniae seropositivity in an SPF breeding herd
title_fullStr Use of trachea-bronchial swab qPCR testing to confirm Mycoplasma hyopneumoniae seropositivity in an SPF breeding herd
title_full_unstemmed Use of trachea-bronchial swab qPCR testing to confirm Mycoplasma hyopneumoniae seropositivity in an SPF breeding herd
title_short Use of trachea-bronchial swab qPCR testing to confirm Mycoplasma hyopneumoniae seropositivity in an SPF breeding herd
title_sort use of trachea-bronchial swab qpcr testing to confirm mycoplasma hyopneumoniae seropositivity in an spf breeding herd
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5984420/
https://www.ncbi.nlm.nih.gov/pubmed/29881637
http://dx.doi.org/10.1186/s40813-018-0088-3
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