Successful Transfer of a Model T-DNA Plasmid to E. coli Revealed Its Dependence on Recipient RecA and the Preference of VirD2 Relaxase for Eukaryotes Rather Than Bacteria as Recipients

In Agrobacterium-mediated transformation (AMT) of plants, a single-strand (ss) T-DNA covalently linked with a VirD2 protein moves through a bacterial type IV secretion channel called VirB/D4. This transport system originates from conjugal plasmid transfer systems of bacteria. The relaxase VirD2 and...

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Detalles Bibliográficos
Autores principales: Ohmine, Yuta, Kiyokawa, Kazuya, Yunoki, Kazuya, Yamamoto, Shinji, Moriguchi, Kazuki, Suzuki, Katsunori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5985610/
https://www.ncbi.nlm.nih.gov/pubmed/29892270
http://dx.doi.org/10.3389/fmicb.2018.00895

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5985610/
https://www.ncbi.nlm.nih.gov/pubmed/29892270
http://dx.doi.org/10.3389/fmicb.2018.00895

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