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Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics
Single-headed myosin 1 has been identified in neurons, but its function in these cells is still unclear. We demonstrate that depletion of myosin 1b (Myo1b), inhibition of its motor activity, or its binding to phosphoinositides impairs the formation of the axon, whereas overexpression of Myo1b increa...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987710/ https://www.ncbi.nlm.nih.gov/pubmed/29588377 http://dx.doi.org/10.1083/jcb.201703205 |
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author | Iuliano, Olga Yoshimura, Azumi Prospéri, Marie-Thérèse Martin, René Knölker, Hans-Joachim Coudrier, Evelyne |
author_facet | Iuliano, Olga Yoshimura, Azumi Prospéri, Marie-Thérèse Martin, René Knölker, Hans-Joachim Coudrier, Evelyne |
author_sort | Iuliano, Olga |
collection | PubMed |
description | Single-headed myosin 1 has been identified in neurons, but its function in these cells is still unclear. We demonstrate that depletion of myosin 1b (Myo1b), inhibition of its motor activity, or its binding to phosphoinositides impairs the formation of the axon, whereas overexpression of Myo1b increases the number of axon-like structures. Myo1b is associated with growth cones and actin waves, two major contributors to neuronal symmetry breaking. We show that Myo1b controls the dynamics of the growth cones and the anterograde propagation of the actin waves. By coupling the membrane to the actin cytoskeleton, Myo1b regulates the size of the actin network as well as the stability and size of filopodia in the growth cones. Our data provide the first evidence that a myosin 1 plays a major role in neuronal symmetry breaking and argue for a mechanical control of the actin cytoskeleton both in actin waves and in the growth cones by this myosin. |
format | Online Article Text |
id | pubmed-5987710 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-59877102018-12-04 Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics Iuliano, Olga Yoshimura, Azumi Prospéri, Marie-Thérèse Martin, René Knölker, Hans-Joachim Coudrier, Evelyne J Cell Biol Research Articles Single-headed myosin 1 has been identified in neurons, but its function in these cells is still unclear. We demonstrate that depletion of myosin 1b (Myo1b), inhibition of its motor activity, or its binding to phosphoinositides impairs the formation of the axon, whereas overexpression of Myo1b increases the number of axon-like structures. Myo1b is associated with growth cones and actin waves, two major contributors to neuronal symmetry breaking. We show that Myo1b controls the dynamics of the growth cones and the anterograde propagation of the actin waves. By coupling the membrane to the actin cytoskeleton, Myo1b regulates the size of the actin network as well as the stability and size of filopodia in the growth cones. Our data provide the first evidence that a myosin 1 plays a major role in neuronal symmetry breaking and argue for a mechanical control of the actin cytoskeleton both in actin waves and in the growth cones by this myosin. Rockefeller University Press 2018-06-04 /pmc/articles/PMC5987710/ /pubmed/29588377 http://dx.doi.org/10.1083/jcb.201703205 Text en © 2018 Iuliano et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Iuliano, Olga Yoshimura, Azumi Prospéri, Marie-Thérèse Martin, René Knölker, Hans-Joachim Coudrier, Evelyne Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics |
title | Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics |
title_full | Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics |
title_fullStr | Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics |
title_full_unstemmed | Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics |
title_short | Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics |
title_sort | myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987710/ https://www.ncbi.nlm.nih.gov/pubmed/29588377 http://dx.doi.org/10.1083/jcb.201703205 |
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