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Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics

Single-headed myosin 1 has been identified in neurons, but its function in these cells is still unclear. We demonstrate that depletion of myosin 1b (Myo1b), inhibition of its motor activity, or its binding to phosphoinositides impairs the formation of the axon, whereas overexpression of Myo1b increa...

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Autores principales: Iuliano, Olga, Yoshimura, Azumi, Prospéri, Marie-Thérèse, Martin, René, Knölker, Hans-Joachim, Coudrier, Evelyne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987710/
https://www.ncbi.nlm.nih.gov/pubmed/29588377
http://dx.doi.org/10.1083/jcb.201703205
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author Iuliano, Olga
Yoshimura, Azumi
Prospéri, Marie-Thérèse
Martin, René
Knölker, Hans-Joachim
Coudrier, Evelyne
author_facet Iuliano, Olga
Yoshimura, Azumi
Prospéri, Marie-Thérèse
Martin, René
Knölker, Hans-Joachim
Coudrier, Evelyne
author_sort Iuliano, Olga
collection PubMed
description Single-headed myosin 1 has been identified in neurons, but its function in these cells is still unclear. We demonstrate that depletion of myosin 1b (Myo1b), inhibition of its motor activity, or its binding to phosphoinositides impairs the formation of the axon, whereas overexpression of Myo1b increases the number of axon-like structures. Myo1b is associated with growth cones and actin waves, two major contributors to neuronal symmetry breaking. We show that Myo1b controls the dynamics of the growth cones and the anterograde propagation of the actin waves. By coupling the membrane to the actin cytoskeleton, Myo1b regulates the size of the actin network as well as the stability and size of filopodia in the growth cones. Our data provide the first evidence that a myosin 1 plays a major role in neuronal symmetry breaking and argue for a mechanical control of the actin cytoskeleton both in actin waves and in the growth cones by this myosin.
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spelling pubmed-59877102018-12-04 Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics Iuliano, Olga Yoshimura, Azumi Prospéri, Marie-Thérèse Martin, René Knölker, Hans-Joachim Coudrier, Evelyne J Cell Biol Research Articles Single-headed myosin 1 has been identified in neurons, but its function in these cells is still unclear. We demonstrate that depletion of myosin 1b (Myo1b), inhibition of its motor activity, or its binding to phosphoinositides impairs the formation of the axon, whereas overexpression of Myo1b increases the number of axon-like structures. Myo1b is associated with growth cones and actin waves, two major contributors to neuronal symmetry breaking. We show that Myo1b controls the dynamics of the growth cones and the anterograde propagation of the actin waves. By coupling the membrane to the actin cytoskeleton, Myo1b regulates the size of the actin network as well as the stability and size of filopodia in the growth cones. Our data provide the first evidence that a myosin 1 plays a major role in neuronal symmetry breaking and argue for a mechanical control of the actin cytoskeleton both in actin waves and in the growth cones by this myosin. Rockefeller University Press 2018-06-04 /pmc/articles/PMC5987710/ /pubmed/29588377 http://dx.doi.org/10.1083/jcb.201703205 Text en © 2018 Iuliano et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Iuliano, Olga
Yoshimura, Azumi
Prospéri, Marie-Thérèse
Martin, René
Knölker, Hans-Joachim
Coudrier, Evelyne
Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics
title Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics
title_full Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics
title_fullStr Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics
title_full_unstemmed Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics
title_short Myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics
title_sort myosin 1b promotes axon formation by regulating actin wave propagation and growth cone dynamics
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987710/
https://www.ncbi.nlm.nih.gov/pubmed/29588377
http://dx.doi.org/10.1083/jcb.201703205
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