High-quality ultrastructural preservation using cryofixation for 3D electron microscopy of genetically labeled tissues

Electron microscopy (EM) offers unparalleled power to study cell substructures at the nanoscale. Cryofixation by high-pressure freezing offers optimal morphological preservation, as it captures cellular structures instantaneously in their near-native state. However, the applicability of cryofixation...

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Detalles Bibliográficos
Autores principales: Tsang, Tin Ki, Bushong, Eric A, Boassa, Daniela, Hu, Junru, Romoli, Benedetto, Phan, Sebastien, Dulcis, Davide, Su, Chih-Ying, Ellisman, Mark H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5988420/
https://www.ncbi.nlm.nih.gov/pubmed/29749931
http://dx.doi.org/10.7554/eLife.35524

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5988420/
https://www.ncbi.nlm.nih.gov/pubmed/29749931
http://dx.doi.org/10.7554/eLife.35524

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