Set up of a protocol for rat plasma peptidomics in hemorrhagic shock model in presence of heparin

A preliminary mass spectrometry based shotgun protocol was set up to compare the peptidome of plasma samples from healthy and hemorrhagic shock rats with the aim of verifying the possible role of uncontrolled proteolytic activity in circulatory shock. Since the hemorrhagic shock model requires hepar...

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Autores principales: Maffioli, Elisa, Aletti, Federico, Santagata, Fabiana, Negri, Armando, Santamaria, Marco H., De Lano, Frank A., Kistler, Erik B., Schmid-Schönbein, Geert W., Tedeschi, Gabriella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Special Section: Proceedings of the 9th Annual EuPA Congress “Proteomics - Back to the Future” (June 23 - 28, 2015, Milano, Italy)
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5988501/
https://www.ncbi.nlm.nih.gov/pubmed/29900112
http://dx.doi.org/10.1016/j.euprot.2016.03.004
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author Maffioli, Elisa
Aletti, Federico
Santagata, Fabiana
Negri, Armando
Santamaria, Marco H.
De Lano, Frank A.
Kistler, Erik B.
Schmid-Schönbein, Geert W.
Tedeschi, Gabriella
author_facet Maffioli, Elisa
Aletti, Federico
Santagata, Fabiana
Negri, Armando
Santamaria, Marco H.
De Lano, Frank A.
Kistler, Erik B.
Schmid-Schönbein, Geert W.
Tedeschi, Gabriella
author_sort Maffioli, Elisa
collection PubMed
description A preliminary mass spectrometry based shotgun protocol was set up to compare the peptidome of plasma samples from healthy and hemorrhagic shock rats with the aim of verifying the possible role of uncontrolled proteolytic activity in circulatory shock. Since the hemorrhagic shock model requires heparin as anticoagulant, a preliminary experiment using plasma sample obtained in the presence/absence of heparin from healthy rats was performed to determine whether its presence is fully compatible with the peptidomic protocol proposed. The entire protocol was tested in a pilot experiment to compare the peptidome of healthy or heparin-anticoagulated rats subjected to hemorrhagic shock.
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spelling pubmed-59885012018-06-13 Set up of a protocol for rat plasma peptidomics in hemorrhagic shock model in presence of heparin Maffioli, Elisa Aletti, Federico Santagata, Fabiana Negri, Armando Santamaria, Marco H. De Lano, Frank A. Kistler, Erik B. Schmid-Schönbein, Geert W. Tedeschi, Gabriella EuPA Open Proteom Special Section: Proceedings of the 9th Annual EuPA Congress “Proteomics - Back to the Future” (June 23 - 28, 2015, Milano, Italy) A preliminary mass spectrometry based shotgun protocol was set up to compare the peptidome of plasma samples from healthy and hemorrhagic shock rats with the aim of verifying the possible role of uncontrolled proteolytic activity in circulatory shock. Since the hemorrhagic shock model requires heparin as anticoagulant, a preliminary experiment using plasma sample obtained in the presence/absence of heparin from healthy rats was performed to determine whether its presence is fully compatible with the peptidomic protocol proposed. The entire protocol was tested in a pilot experiment to compare the peptidome of healthy or heparin-anticoagulated rats subjected to hemorrhagic shock. Elsevier 2016-03-16 /pmc/articles/PMC5988501/ /pubmed/29900112 http://dx.doi.org/10.1016/j.euprot.2016.03.004 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Special Section: Proceedings of the 9th Annual EuPA Congress “Proteomics - Back to the Future” (June 23 - 28, 2015, Milano, Italy)
Maffioli, Elisa
Aletti, Federico
Santagata, Fabiana
Negri, Armando
Santamaria, Marco H.
De Lano, Frank A.
Kistler, Erik B.
Schmid-Schönbein, Geert W.
Tedeschi, Gabriella
Set up of a protocol for rat plasma peptidomics in hemorrhagic shock model in presence of heparin
title Set up of a protocol for rat plasma peptidomics in hemorrhagic shock model in presence of heparin
title_full Set up of a protocol for rat plasma peptidomics in hemorrhagic shock model in presence of heparin
title_fullStr Set up of a protocol for rat plasma peptidomics in hemorrhagic shock model in presence of heparin
title_full_unstemmed Set up of a protocol for rat plasma peptidomics in hemorrhagic shock model in presence of heparin
title_short Set up of a protocol for rat plasma peptidomics in hemorrhagic shock model in presence of heparin
title_sort set up of a protocol for rat plasma peptidomics in hemorrhagic shock model in presence of heparin
topic Special Section: Proceedings of the 9th Annual EuPA Congress “Proteomics - Back to the Future” (June 23 - 28, 2015, Milano, Italy)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5988501/
https://www.ncbi.nlm.nih.gov/pubmed/29900112
http://dx.doi.org/10.1016/j.euprot.2016.03.004
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