Experimental setup for the identification of mitochondrial protease substrates by shotgun and top-down proteomics

Mitochondria possess a proteolytic system that contributes to the regulation of mitochondrial dynamics, mitochondrial biogenesis and mitophagy. We aimed at the identification by bottom-up proteomics of altered protein processing due to the activation of mitochondrial proteases in a cellular model of...

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Autores principales: Di Pierro, Alice, Bondi, Heather, Monti, Chiara, Pieroni, Luisa, Cilio, Enrico, Urbani, Andrea, Alberio, Tiziana, Fasano, Mauro, Ronci, Maurizio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Special Section: Proceedings of the 9th Annual EuPA Congress “Proteomics - Back to the Future” (June 23 - 28, 2015, Milano, Italy)
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5988556/
https://www.ncbi.nlm.nih.gov/pubmed/29900104
http://dx.doi.org/10.1016/j.euprot.2016.02.002
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author Di Pierro, Alice
Bondi, Heather
Monti, Chiara
Pieroni, Luisa
Cilio, Enrico
Urbani, Andrea
Alberio, Tiziana
Fasano, Mauro
Ronci, Maurizio
author_facet Di Pierro, Alice
Bondi, Heather
Monti, Chiara
Pieroni, Luisa
Cilio, Enrico
Urbani, Andrea
Alberio, Tiziana
Fasano, Mauro
Ronci, Maurizio
author_sort Di Pierro, Alice
collection PubMed
description Mitochondria possess a proteolytic system that contributes to the regulation of mitochondrial dynamics, mitochondrial biogenesis and mitophagy. We aimed at the identification by bottom-up proteomics of altered protein processing due to the activation of mitochondrial proteases in a cellular model of impaired dopamine homeostasis. Moreover, we optimized the conditions for top-down proteomics to identify the cleavage site sequences.
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spelling pubmed-59885562018-06-13 Experimental setup for the identification of mitochondrial protease substrates by shotgun and top-down proteomics Di Pierro, Alice Bondi, Heather Monti, Chiara Pieroni, Luisa Cilio, Enrico Urbani, Andrea Alberio, Tiziana Fasano, Mauro Ronci, Maurizio EuPA Open Proteom Special Section: Proceedings of the 9th Annual EuPA Congress “Proteomics - Back to the Future” (June 23 - 28, 2015, Milano, Italy) Mitochondria possess a proteolytic system that contributes to the regulation of mitochondrial dynamics, mitochondrial biogenesis and mitophagy. We aimed at the identification by bottom-up proteomics of altered protein processing due to the activation of mitochondrial proteases in a cellular model of impaired dopamine homeostasis. Moreover, we optimized the conditions for top-down proteomics to identify the cleavage site sequences. Elsevier 2016-02-22 /pmc/articles/PMC5988556/ /pubmed/29900104 http://dx.doi.org/10.1016/j.euprot.2016.02.002 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Special Section: Proceedings of the 9th Annual EuPA Congress “Proteomics - Back to the Future” (June 23 - 28, 2015, Milano, Italy)
Di Pierro, Alice
Bondi, Heather
Monti, Chiara
Pieroni, Luisa
Cilio, Enrico
Urbani, Andrea
Alberio, Tiziana
Fasano, Mauro
Ronci, Maurizio
Experimental setup for the identification of mitochondrial protease substrates by shotgun and top-down proteomics
title Experimental setup for the identification of mitochondrial protease substrates by shotgun and top-down proteomics
title_full Experimental setup for the identification of mitochondrial protease substrates by shotgun and top-down proteomics
title_fullStr Experimental setup for the identification of mitochondrial protease substrates by shotgun and top-down proteomics
title_full_unstemmed Experimental setup for the identification of mitochondrial protease substrates by shotgun and top-down proteomics
title_short Experimental setup for the identification of mitochondrial protease substrates by shotgun and top-down proteomics
title_sort experimental setup for the identification of mitochondrial protease substrates by shotgun and top-down proteomics
topic Special Section: Proceedings of the 9th Annual EuPA Congress “Proteomics - Back to the Future” (June 23 - 28, 2015, Milano, Italy)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5988556/
https://www.ncbi.nlm.nih.gov/pubmed/29900104
http://dx.doi.org/10.1016/j.euprot.2016.02.002
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