Solubilization and renaturation of biologically active human bone morphogenetic protein-4 from inclusion bodies

Biologically active human bone morphogenetic protein-4 (hBMP-4) was successfully produced in a prokaryotic host. For this aim, hBMP-4 cDNA was cloned in Escherichia coli (E. coli) and the protein was produced in a non-active aggregated form. After washing and solubilization, in vitro refolding of th...

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Detalles Bibliográficos
Autores principales: Gieseler, Gesa-Maria, Ekramzadeh, Kimia, Nölle, Volker, Malysheva, Svitlana, Kempf, Henning, Beutel, Sascha, Zweigerdt, Robert, Martin, Ulrich, Rinas, Ursula, Scheper, Thomas, Pepelanova, Iliyana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989590/
https://www.ncbi.nlm.nih.gov/pubmed/29876300
http://dx.doi.org/10.1016/j.btre.2018.e00249

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989590/
https://www.ncbi.nlm.nih.gov/pubmed/29876300
http://dx.doi.org/10.1016/j.btre.2018.e00249

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