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Solubilization and renaturation of biologically active human bone morphogenetic protein-4 from inclusion bodies
Biologically active human bone morphogenetic protein-4 (hBMP-4) was successfully produced in a prokaryotic host. For this aim, hBMP-4 cDNA was cloned in Escherichia coli (E. coli) and the protein was produced in a non-active aggregated form. After washing and solubilization, in vitro refolding of th...
Autores principales: | Gieseler, Gesa-Maria, Ekramzadeh, Kimia, Nölle, Volker, Malysheva, Svitlana, Kempf, Henning, Beutel, Sascha, Zweigerdt, Robert, Martin, Ulrich, Rinas, Ursula, Scheper, Thomas, Pepelanova, Iliyana |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989590/ https://www.ncbi.nlm.nih.gov/pubmed/29876300 http://dx.doi.org/10.1016/j.btre.2018.e00249 |
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