In vitro synthesis of phospholipids with yeast phospholipase B, a phospholipid deacylating enzyme

The gene encoding the Saccharomyces cerevisiae phospholipid deacylation enzyme, phospholipase B (ScPLB1), was successfully expressed in E. coli. The enzyme (Scplb1p) was engineered to have a histidine-tag at the C-terminal end and was purified by metal (Ni) affinity chromatography. Enzymatic propert...

Descripción completa

Detalles Bibliográficos
Autores principales: Watanabe, Yasuo, Kobayashi, Itsuki, Ohnaka, Takanori, Watanabe, Seiya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989593/
https://www.ncbi.nlm.nih.gov/pubmed/29876301
http://dx.doi.org/10.1016/j.btre.2018.e00250

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989593/
https://www.ncbi.nlm.nih.gov/pubmed/29876301
http://dx.doi.org/10.1016/j.btre.2018.e00250

Ejemplares similares