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In vitro synthesis of phospholipids with yeast phospholipase B, a phospholipid deacylating enzyme
The gene encoding the Saccharomyces cerevisiae phospholipid deacylation enzyme, phospholipase B (ScPLB1), was successfully expressed in E. coli. The enzyme (Scplb1p) was engineered to have a histidine-tag at the C-terminal end and was purified by metal (Ni) affinity chromatography. Enzymatic propert...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989593/ https://www.ncbi.nlm.nih.gov/pubmed/29876301 http://dx.doi.org/10.1016/j.btre.2018.e00250 |