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Deacetylation of metabolic enzymes by Sirt2 modulates pyruvate homeostasis to extend insect lifespan
Diapause in insects is akin to dauer in Caenorhabditis elegans and hibernation in vertebrates. Diapause causes a profound extension of lifespan by low metabolic activity. However, the detailed regulatory mechanisms for low metabolic activity remain unknown. Here, we showed that low pyruvate levels a...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5990394/ https://www.ncbi.nlm.nih.gov/pubmed/29769432 http://dx.doi.org/10.18632/aging.101447 |
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author | Wang, Tao Geng, Shao-Lei Guan, Yu-Min Xu, Wei-Hua |
author_facet | Wang, Tao Geng, Shao-Lei Guan, Yu-Min Xu, Wei-Hua |
author_sort | Wang, Tao |
collection | PubMed |
description | Diapause in insects is akin to dauer in Caenorhabditis elegans and hibernation in vertebrates. Diapause causes a profound extension of lifespan by low metabolic activity. However, the detailed regulatory mechanisms for low metabolic activity remain unknown. Here, we showed that low pyruvate levels are present in the brains of diapause-destined pupae of the cotton bollworm Helicoverpa armigera, and three enzymes pyruvate kinase (PK), phosphoenolpyruvate carboxykinase (PEPCK), and phosphoglycerate mutase (PGAM) are closely correlated with pyruvate homeostasis. Notably, Sirt2 can deacetylate the three enzymes to increase their activity in vitro. Thus, low Sirt2 expression in the brains of diapause individuals decreases PK and PEPCK protein levels as well as PGAM activity, resulting in low pyruvate levels and low tricarboxylic acid cycle activity and eventually inducing diapause initiation by low metabolic activity. These findings suggest that pyruvate is a checkpoint for development or lifespan extension, and Sirt2 is a negative regulator to extend lifespan in insects. |
format | Online Article Text |
id | pubmed-5990394 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-59903942018-06-07 Deacetylation of metabolic enzymes by Sirt2 modulates pyruvate homeostasis to extend insect lifespan Wang, Tao Geng, Shao-Lei Guan, Yu-Min Xu, Wei-Hua Aging (Albany NY) Research Paper Diapause in insects is akin to dauer in Caenorhabditis elegans and hibernation in vertebrates. Diapause causes a profound extension of lifespan by low metabolic activity. However, the detailed regulatory mechanisms for low metabolic activity remain unknown. Here, we showed that low pyruvate levels are present in the brains of diapause-destined pupae of the cotton bollworm Helicoverpa armigera, and three enzymes pyruvate kinase (PK), phosphoenolpyruvate carboxykinase (PEPCK), and phosphoglycerate mutase (PGAM) are closely correlated with pyruvate homeostasis. Notably, Sirt2 can deacetylate the three enzymes to increase their activity in vitro. Thus, low Sirt2 expression in the brains of diapause individuals decreases PK and PEPCK protein levels as well as PGAM activity, resulting in low pyruvate levels and low tricarboxylic acid cycle activity and eventually inducing diapause initiation by low metabolic activity. These findings suggest that pyruvate is a checkpoint for development or lifespan extension, and Sirt2 is a negative regulator to extend lifespan in insects. Impact Journals 2018-05-16 /pmc/articles/PMC5990394/ /pubmed/29769432 http://dx.doi.org/10.18632/aging.101447 Text en Copyright © 2018 Wang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Wang, Tao Geng, Shao-Lei Guan, Yu-Min Xu, Wei-Hua Deacetylation of metabolic enzymes by Sirt2 modulates pyruvate homeostasis to extend insect lifespan |
title | Deacetylation of metabolic enzymes by Sirt2 modulates pyruvate homeostasis to extend insect lifespan |
title_full | Deacetylation of metabolic enzymes by Sirt2 modulates pyruvate homeostasis to extend insect lifespan |
title_fullStr | Deacetylation of metabolic enzymes by Sirt2 modulates pyruvate homeostasis to extend insect lifespan |
title_full_unstemmed | Deacetylation of metabolic enzymes by Sirt2 modulates pyruvate homeostasis to extend insect lifespan |
title_short | Deacetylation of metabolic enzymes by Sirt2 modulates pyruvate homeostasis to extend insect lifespan |
title_sort | deacetylation of metabolic enzymes by sirt2 modulates pyruvate homeostasis to extend insect lifespan |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5990394/ https://www.ncbi.nlm.nih.gov/pubmed/29769432 http://dx.doi.org/10.18632/aging.101447 |
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