High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR/Cas9 in yeast

Construction and characterization of large genetic variant libraries is essential for understanding genome function, but remains challenging. Here, we introduce a Cas9-based approach for generating pools of mutants with defined genetic alterations (deletions, substitutions, and insertions) with an e...

Descripción completa

Detalles Bibliográficos
Autores principales: Guo, Xiaoge, Chavez, Alejandro, Tung, Angela, Chan, Yingleong, Kaas, Christian, Yin, Yi, Cecchi, Ryan, Garnier, Santiago Lopez, Kelsic, Eric, Schubert, Max, DiCarlo, James E., Collins, James J., Church, George M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5990468/
https://www.ncbi.nlm.nih.gov/pubmed/29786095
http://dx.doi.org/10.1038/nbt.4147

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5990468/
https://www.ncbi.nlm.nih.gov/pubmed/29786095
http://dx.doi.org/10.1038/nbt.4147

Ejemplares similares