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Far rapid synthesis of giant DNA in the Bacillus subtilis genome by a conjugation transfer system
Bacillus subtilis offers a platform for giant DNA synthesis, which is mediated by the connection of overlapping DNA segments called domino DNA, in the cloning locus of the host. The domino method was successfully used to produce DNA fragments as large as 3500 kbp. However, domino DNA is limited to &...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5993740/ https://www.ncbi.nlm.nih.gov/pubmed/29884789 http://dx.doi.org/10.1038/s41598-018-26987-0 |