Restriction digest screening facilitates efficient detection of site-directed mutations introduced by CRISPR in C. albicans UME6

Introduction of point mutations to a gene of interest is a powerful tool when determining protein function. CRISPR-mediated genome editing allows for more efficient transfer of a desired mutation into a wide range of model organisms. Traditionally, PCR amplification and DNA sequencing is used to det...

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Detalles Bibliográficos
Autores principales: Evans, Ben A., Smith, Olivia L., Pickerill, Ethan S., York, Mary K., Buenconsejo, Kristen J.P., Chambers, Antonio E., Bernstein, Douglas A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Cell Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994162/
https://www.ncbi.nlm.nih.gov/pubmed/29892505
http://dx.doi.org/10.7717/peerj.4920

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994162/
https://www.ncbi.nlm.nih.gov/pubmed/29892505
http://dx.doi.org/10.7717/peerj.4920

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