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Restriction digest screening facilitates efficient detection of site-directed mutations introduced by CRISPR in C. albicans UME6
Introduction of point mutations to a gene of interest is a powerful tool when determining protein function. CRISPR-mediated genome editing allows for more efficient transfer of a desired mutation into a wide range of model organisms. Traditionally, PCR amplification and DNA sequencing is used to det...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
PeerJ Inc.
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994162/ https://www.ncbi.nlm.nih.gov/pubmed/29892505 http://dx.doi.org/10.7717/peerj.4920 |