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A Novel Ultrasensitive In Situ Hybridization Approach to Detect Short Sequences and Splice Variants with Cellular Resolution

Investigating the expression of RNAs that differ by short or single nucleotide sequences at a single-cell level in tissue has been limited by the sensitivity and specificity of in situ hybridization (ISH) techniques. Detection of short isoform-specific sequences requires RNA isolation for PCR analys...

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Detalles Bibliográficos
Autores principales: Erben, Larissa, He, Ming-Xiao, Laeremans, Annelies, Park, Emily, Buonanno, Andres
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994223/
https://www.ncbi.nlm.nih.gov/pubmed/29264769
http://dx.doi.org/10.1007/s12035-017-0834-6