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A Novel Ultrasensitive In Situ Hybridization Approach to Detect Short Sequences and Splice Variants with Cellular Resolution
Investigating the expression of RNAs that differ by short or single nucleotide sequences at a single-cell level in tissue has been limited by the sensitivity and specificity of in situ hybridization (ISH) techniques. Detection of short isoform-specific sequences requires RNA isolation for PCR analys...
Autores principales: | Erben, Larissa, He, Ming-Xiao, Laeremans, Annelies, Park, Emily, Buonanno, Andres |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994223/ https://www.ncbi.nlm.nih.gov/pubmed/29264769 http://dx.doi.org/10.1007/s12035-017-0834-6 |
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