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An Efficient Protocol for Model Legume Root Protoplast Isolation and Transformation

Transient gene expression systems using protoplasts have been widely used for rapid functional characterization of genes and high-throughput analysis in many model and crop species. Here, we describe a simplified and highly efficient root protoplast isolation and transient expression system in the m...

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Detalles Bibliográficos
Autores principales: Jia, Ning, Zhu, Yali, Xie, Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994418/
https://www.ncbi.nlm.nih.gov/pubmed/29915605
http://dx.doi.org/10.3389/fpls.2018.00670