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Controlling Ethanol Use in Chain Elongation by CO(2) Loading Rate

[Image: see text] Chain elongation is an open-culture biotechnological process which converts volatile fatty acids (VFAs) into medium chain fatty acids (MCFAs) using ethanol and other reduced substrates. The objective of this study was to investigate the quantitative effect of CO(2) loading rate on...

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Autores principales: Roghair, Mark, Hoogstad, Tim, Strik, David P.B.T.B., Plugge, Caroline M., Timmers, Peer H.A., Weusthuis, Ruud A., Bruins, Marieke E., Buisman, Cees J. N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5997387/
https://www.ncbi.nlm.nih.gov/pubmed/29304274
http://dx.doi.org/10.1021/acs.est.7b04904
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author Roghair, Mark
Hoogstad, Tim
Strik, David P.B.T.B.
Plugge, Caroline M.
Timmers, Peer H.A.
Weusthuis, Ruud A.
Bruins, Marieke E.
Buisman, Cees J. N.
author_facet Roghair, Mark
Hoogstad, Tim
Strik, David P.B.T.B.
Plugge, Caroline M.
Timmers, Peer H.A.
Weusthuis, Ruud A.
Bruins, Marieke E.
Buisman, Cees J. N.
author_sort Roghair, Mark
collection PubMed
description [Image: see text] Chain elongation is an open-culture biotechnological process which converts volatile fatty acids (VFAs) into medium chain fatty acids (MCFAs) using ethanol and other reduced substrates. The objective of this study was to investigate the quantitative effect of CO(2) loading rate on ethanol usages in a chain elongation process. We supplied different rates of CO(2) to a continuously stirred anaerobic reactor, fed with ethanol and propionate. Ethanol was used to upgrade ethanol itself into caproate and to upgrade the supplied VFA (propionate) into heptanoate. A high CO(2) loading rate (2.5 L(CO2)·L(–1)·d(–1)) stimulated excessive ethanol oxidation (EEO; up to 29%) which resulted in a high caproate production (10.8 g·L(–1)·d(–1)). A low CO(2) loading rate (0.5 L(CO2)·L(–1)·d(–1)) reduced EEO (16%) and caproate production (2.9 g·L(–1)·d(–1)). Heptanoate production by VFA upgrading remained constant (∼1.8 g·L(–1)·d(–1)) at CO(2) loading rates higher than or equal to 1 L(CO2)·L(–1)·d(–1). CO(2) was likely essential for growth of chain elongating microorganisms while it also stimulated syntrophic ethanol oxidation. A high CO(2) loading rate must be selected to upgrade ethanol (e.g., from lignocellulosic bioethanol) into MCFAs whereas lower CO(2) loading rates must be selected to upgrade VFAs (e.g., from acidified organic residues) into MCFAs while minimizing use of costly ethanol.
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spelling pubmed-59973872018-06-13 Controlling Ethanol Use in Chain Elongation by CO(2) Loading Rate Roghair, Mark Hoogstad, Tim Strik, David P.B.T.B. Plugge, Caroline M. Timmers, Peer H.A. Weusthuis, Ruud A. Bruins, Marieke E. Buisman, Cees J. N. Environ Sci Technol [Image: see text] Chain elongation is an open-culture biotechnological process which converts volatile fatty acids (VFAs) into medium chain fatty acids (MCFAs) using ethanol and other reduced substrates. The objective of this study was to investigate the quantitative effect of CO(2) loading rate on ethanol usages in a chain elongation process. We supplied different rates of CO(2) to a continuously stirred anaerobic reactor, fed with ethanol and propionate. Ethanol was used to upgrade ethanol itself into caproate and to upgrade the supplied VFA (propionate) into heptanoate. A high CO(2) loading rate (2.5 L(CO2)·L(–1)·d(–1)) stimulated excessive ethanol oxidation (EEO; up to 29%) which resulted in a high caproate production (10.8 g·L(–1)·d(–1)). A low CO(2) loading rate (0.5 L(CO2)·L(–1)·d(–1)) reduced EEO (16%) and caproate production (2.9 g·L(–1)·d(–1)). Heptanoate production by VFA upgrading remained constant (∼1.8 g·L(–1)·d(–1)) at CO(2) loading rates higher than or equal to 1 L(CO2)·L(–1)·d(–1). CO(2) was likely essential for growth of chain elongating microorganisms while it also stimulated syntrophic ethanol oxidation. A high CO(2) loading rate must be selected to upgrade ethanol (e.g., from lignocellulosic bioethanol) into MCFAs whereas lower CO(2) loading rates must be selected to upgrade VFAs (e.g., from acidified organic residues) into MCFAs while minimizing use of costly ethanol. American Chemical Society 2018-01-05 2018-02-06 /pmc/articles/PMC5997387/ /pubmed/29304274 http://dx.doi.org/10.1021/acs.est.7b04904 Text en Copyright © 2018 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Roghair, Mark
Hoogstad, Tim
Strik, David P.B.T.B.
Plugge, Caroline M.
Timmers, Peer H.A.
Weusthuis, Ruud A.
Bruins, Marieke E.
Buisman, Cees J. N.
Controlling Ethanol Use in Chain Elongation by CO(2) Loading Rate
title Controlling Ethanol Use in Chain Elongation by CO(2) Loading Rate
title_full Controlling Ethanol Use in Chain Elongation by CO(2) Loading Rate
title_fullStr Controlling Ethanol Use in Chain Elongation by CO(2) Loading Rate
title_full_unstemmed Controlling Ethanol Use in Chain Elongation by CO(2) Loading Rate
title_short Controlling Ethanol Use in Chain Elongation by CO(2) Loading Rate
title_sort controlling ethanol use in chain elongation by co(2) loading rate
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5997387/
https://www.ncbi.nlm.nih.gov/pubmed/29304274
http://dx.doi.org/10.1021/acs.est.7b04904
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