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Optimizing CRISPR/Cas9 for the Diatom Phaeodactylum tricornutum
CRISPR/Cas9 is a powerful tool for genome editing. We constructed an easy-to-handle expression vector for application in the model organism Phaeodactylum tricornutum and tested its capabilities in order to apply CRISPR/Cas9 technology for our purpose. In our experiments, we targeted two different ge...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5998643/ https://www.ncbi.nlm.nih.gov/pubmed/29928285 http://dx.doi.org/10.3389/fpls.2018.00740 |