Mammalian expression vectors for metabolic biotinylation tandem affinity tagging by co-expression in cis of a mammalian codon-optimized BirA biotin ligase

ΟBJECTIVE: To construct mammalian expression vectors for the N- or C-terminal tagging of proteins with a tandem affinity tag comprised of the biotinylatable Avi tag and of a triple FLAG tag. RESULTS: We constructed and tested by transient transfections mammalian expression vectors for the co-express...

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Detalles Bibliográficos
Autores principales: Ioannou, Marina, Papageorgiou, Dimitris N., Ogryzko, Vasily, Strouboulis, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001059/
https://www.ncbi.nlm.nih.gov/pubmed/29898783
http://dx.doi.org/10.1186/s13104-018-3500-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001059/
https://www.ncbi.nlm.nih.gov/pubmed/29898783
http://dx.doi.org/10.1186/s13104-018-3500-9

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